DreINT0150008 @ danRer10
Intron Retention
Gene
ENSDARG00000035468 | slc25a25b
Description
solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25b [Source:ZFIN;Acc:ZDB-GENE-060526-340]
Coordinates
chr5:31193371-31195546:+
Coord C1 exon
chr5:31193371-31193497
Coord A exon
chr5:31193498-31195458
Coord C2 exon
chr5:31195459-31195546
Length
1961 bp
Sequences
Splice sites
5' ss Seq
ATGGTAAGA
5' ss Score
9.48
3' ss Seq
TTCTCCTTTTAAATCTGCAGGTC
3' ss Score
10.52
Exon sequences
Seq C1 exon
AAAGTCGTCAAGGCTGGAGACAAAGACCTGGATGGTCAGTTGGACTTTGAGGAGTTTGTGCACTACCTGAGGGACCACGAAAAGAAACTCAGGCTGGTCTTCAAGAGTCTTGATAAAAAGAACGATG
Seq A exon
GTAAGAATTTTTTAGTCAACTACACCGGAATTCGAATGTGTGAATCTGTTGCTAACTTTACTGAACTTCAACACTTGACATTTTTACCGCAGACCGGGGTGGAGGTTGTGGTTAGAGTTACCAATAAACCATTTTCTCTGAGGATGACAAGCTGACAAAACATTGTTGTCACGCTGATACAAGTTTTATTTATGGAAAAAAATACAAAGCACTGCTGTGTTTGGGTGTTCTGTGTTTGTCTACTGAAATGTGTATATTCTATACAAATTGAAGCTGATCAGTCTATTCTTGTCACGTGGCTCCCAGTTCGCCTGCAGGATGCATTCAACAAGCCGCGCACCTCCATTGGAACCCATGAACTTGTGCAAACTCTAGAAAAGACGCAATATCCAAACGTCATTTGCGATCTCTATCATTTGATCTTGCACAGACATTGTGGATTCACCTGGTTTGTTGACAAATGGGTTGTGGATTCATTCCTTGGCAGTTCATGGGTCGTTCACTGGGCCTTTTTACCTTTGCAAAGGAACTTTCCGAAGACGTCTGTTTCTTAGGGGCCGATCACACCGAACACGCTTTTATGTTCCAAAAACGCAAGGCGCAGCTCACTGCTTTTTTTGTTGACAAGAAAAAAGAACCGCTGGGTGCAAAAGTGTTGCTGTTTATATTAATATAATTTTAGTATTTAATAATACTGTGATATTCTAGACTTGTGAAGTCATAGAGCTCCTGATAACTCAAAACAACAACCACAAGTCTCTCCTCCATATTCAAAAGTCTTAGTTGTTGTCTTGACAACACAAACATTGCACCTCAACGAAAACCTTACCTCTGCTTTCATTTGGAGAATGAAAAAGACGCGAGTGATGTAACGCACTTCTTTCACTCAGAGCTGACTTTTTTTTCCTCAACTGCGAGCCCATCGCGCACGAGCGAAAACGGGAGGCATAGCAGGCAGTTAAAATATGAGGCGCAAAGGGTGCATAAGCAGCGCACAAAATGTTCGCGGCCGTTAGTAAACCATTTAAAAAAGGTTTAGTGTGAACGACCCCTTACTCATTTTGCTGCTTGTGGGTTAAATTTGGGCACTCAGGTGATCAAGATATAACCTAGAGAATACAGTTATTTTAAAAGATTAAAGATCGTTCAGATTTGGATAATAAATAAAACTAAAAATAATTAATGATTTTTTTGTGCAGCCTCATACCAATTGATCCACGGACCAGTACTGGCTTGCAACTTGGTGGGTCGTTGCTAGATCAGATATAGTTGCGGCCGGAAGTTAACGAAAATTATTTCTAATATTTAATAAATTACCCAATAAATTGTATTTTTTAATGGCTGCCCCAACCCTAAACCCAATCGTCACAGTACTGTAAAATGATTAATTATTGTTATACAGTGTCATAAAAATGTTGCTATATTGATGTCCGTATCGCACTTCTGGCCAGCCGCATATCCGATCTAGACTTTAGCGGCCCAGTCGTTGAGGACCACTGAACTAAACGACAAGCTTGCAGAAAATACCACGTGATATAGTCACAACCAAGCTGCACATTTTCAACACTGGTTTTATCTTGTGGTTTTACACTGGTTTTTACTGGGCTTATGACTTTTGCAGGAATTAATTAACTGTAATAATTAACAGGAACTAATTATGGATATCAATAAAATAACTAAACAATGTGCTTTTTAAAAGTTATGCTACCACATATACCCAGTTTTTCCAAGACAGTGCAAATGCTTTTGGGGGATTATTTAAAGAAAATATTTAATATTTAAACATTAGATGATTTTATTTGTATTTTATTTGTACAGTTTCATTGTTTTTCAAAGTTTGAAAGTGTTTGTGTATATATTCTTTACTTGTTTTGTCACCTTCTTAAGCCACCTTAGAAGTTTTAATTGTTAAATTGATAAAAAAGACAATTGTTTTAACATTTCTCCTTTTAAATCTGCAG
Seq C2 exon
GTCACATTGATTCGCAGGAGATCATGCAGTCACTACGAGACCTTGGGGTTCATATATCTGAGGAACAGGCTGAGAAGATTCTCAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035468:ENSDART00000098163:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.008 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0003627=EF-hand_1=PD(92.6=58.1),PF134991=EF-hand_7=PU(29.9=46.5),PF118483=DUF3368=PU(15.8=14.0)
A:
NA
C2:
PF134991=EF-hand_7=FE(43.3=100),PF118483=DUF3368=FE(76.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGTCGTCAAGGCTGGAGAC
R:
ATCTTCTCAGCCTGTTCCTCAGA
Band lengths:
206-2167
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]