DreINT0171862 @ danRer10
Intron Retention
Gene
ENSDARG00000033327 | unc5b
Description
unc-5 homolog B (C. elegans) [Source:ZFIN;Acc:ZDB-GENE-041213-1]
Coordinates
chr13:29068436-29070434:+
Coord C1 exon
chr13:29068436-29068616
Coord A exon
chr13:29068617-29070266
Coord C2 exon
chr13:29070267-29070434
Length
1650 bp
Sequences
Splice sites
5' ss Seq
ACGGTAACC
5' ss Score
6.62
3' ss Seq
CTCTGGCTCTCTGTTATTAGTAA
3' ss Score
5.61
Exon sequences
Seq C1 exon
GTGGACTGGCTAAAGAACGAAGAGCTCATTGACCCGGCGCTGGATTCTAACTTTCTAATTACCATCGAGCACGACCTGATCATCAAACAGGCTCGACTCTCTGACACTGCCAACTACACCTGTGTGGCACGTAATGTGGTCGCTAAGAGACGCAGCAGCACTGCCACTCTCATCGTCTACG
Seq A exon
GTAACCACTTGACCAATTGCAGTCCCTGCATCAGTCTGAGTCACTGAGTGCACAAGTCAAGGGCTAATTCGGAAAACAGGAGAACTACAATCACCACTCGAGTTTATGAATATTTATAACATGGCTATCTGAAATACTCGCTACTTATTGGTCAGCTCTGGTATTCGACAGTCTGTAATGGTTGTTGTATTAATATATGACTATTTGCCTACTTGTGCTTATATAAATATACACTCACAGAGTTGAAATTGAAACTGATGTTAAATTGTAGTTCTTTTTCAGATATTTCCAAGTGATTTTTAACATATCAAGGAGATTTTTATCAGAATTTTCTTTAATATTATTATATATTTAGTGAGTATTTCCTATAAATAAATTAAGACTTTAAATTGCACTTTAAGCTGAATACTAGTATCTTGCAAATGACTTATAAAACGTTATCTACTGACTCTTAGAAAATATTTGAATATTTTATTTTATTTTATTTTTTTGTATTTAAGACTAATTTAAAAGTAAATTAGCAAAGTTGTGATTTTTGAGTGCACAAAATTTTAGAATATATTATTGAATTGTTTATTTAATTTTTCTTTACTTTGCCTTAAAAATATCTTTTAAAAGTCTTGTATTTACTGTATTTTTTAACAAACAAATGAGTTTCACTTTATTTTGTAGGTCCCTTTAATGCATTTTGTTGAATTTAAGTTACATTGCATTTACATGCCAACTAATTCTCAGTAGTTTATAAGTAGACTGTTAGGTTGGGGTTAGGTTTAGTGTAGGTTGACATGTGCTTGCAAAGTTTCTTATAGTCAGTTAAATGTCTGTTAAAGGAGCAGTAACAACAGATATTAAGCAGACAGTCTACTAATACTCAAATAATAAGTAGTTGCAATGCAGCTTTTAGTCAACAAAATATGCAATAGGGACCATTAAAATAAAGTCTTAGCAATAAATGCAGTTTGAGGTGAGCATAAAAAACACTTTTTCAAAAATGACAACAAAACAACAAAATCCATTTTATTTACAGAATAGATTATATGAACACAAATTTATTCATTGTTCATTCATTATTTAAAATGTTTATTTAATAGTTTGCTATGTTACATGGTGTTTAGTGCATAAATAGTGGTAATAATTGTAATAAAATACATAAATCTTCCTGTCAGAGGTTTATACAGTATTCTTATAATGACCAACAGTATGATATCCCTCAATTAATGCACAACGTGTTTACTTTTGTATTCAGACTTGTGTTAAATTTTTATTCTTTCATTTTTCATTGACTGTTTCAAACATGCTCCATTCTATTCTAATTAAACTGCATGCCAATGTAAACATATCTGATTAATATCTGATCCTGTATTAATAATATGTGTCTCTGGCTGTCTGGTATTAGTAAAAATCCTTCAAATATGACACAAAGGGGTTACTTTTGCATGCTTGCTTGTATTTGTTTTTTATTTTTCTCATTTTCCAAACACTATTTCAAACATTCTCCCTTCTCGTCAGATCTCATCGATTGCCTAAATTCACTGAGAAATGGATAAAAATATTCATTTTCAAAATGGGGTGTACTCAATTATACTGAGCACTGTACACATTCTTGATAAGCTGTATTAATAGTATTTGTCTCTGGCTCTCTGTTATTAG
Seq C2 exon
TAAGTGGAGGCTGGTCATCCTGGACAGAGTGGTCAGAATGTAATGCTCAGTGTGGGCGGGGCTGGCAGAGACGGACACGCAGCTGCACCAATCCAGCACCACTCAATGGAGGAGCCTTCTGTGATGGACCGCCCTTCCAGAGAGTCACCTGTACCACCCTCTGTCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000033327:ENSDART00000045951:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.049 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0767911=I-set=PD(64.8=96.7)
A:
NA
C2:
PF0009014=TSP_1=PU(94.3=89.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGGCTAAAGAACGAAGAGCT
R:
GACAGAGGGTGGTACAGGTGA
Band lengths:
342-1992
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]