DreINT0173887 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24605981-24606308:-
Coord C1 exon
chr2:24606182-24606308
Coord A exon
chr2:24606100-24606181
Coord C2 exon
chr2:24605981-24606099
Length
82 bp
Sequences
Splice sites
5' ss Seq
ATGGTAAGA
5' ss Score
9.48
3' ss Seq
ATACATGATCATACGTTTAGGTG
3' ss Score
3.61
Exon sequences
Seq C1 exon
GCAAATCTTGAGAAGATGACCAGATCTTTAGAAGACCAAATGAATGAATATAAAACAAAATATGAGGAAGGTCAGCGCTGCATTAATGACTTCACAATGCAGAAATCTAAACTACAATCTGAAAATG
Seq A exon
GTAAGATTAGTAAATAACATTCAATTAAATTATTTGAGCAACAGACCTCCAACAATGATAAAATACATGATCATACGTTTAG
Seq C2 exon
GTGAACTTTCAAGACAGCTGGAGGAAAAGGACTCTCTTGTCTCCCAGCTAACCAGAAGCAAGATGTCTTACACTCAGCAAATTGAAGATCTTAAAAGACAACTGGAGGAGGAAACAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:27
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.767 A=NA C2=0.850
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.9=100),PF0003816=Filament=FE(36.8=100),PF059116=DUF869=PU(38.6=90.7),PF0003816=Filament=PU(3.0=23.3)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=FE(34.2=100),PF059116=DUF869=FE(38.6=100),PF0003816=Filament=FE(11.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAGGTCAGCGCTGCATTAA
R:
TGTTTCCTCCTCCAGTTGTCT
Band lengths:
178-260
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]