DreINT0173897 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24602011-24602322:-
Coord C1 exon
chr2:24602227-24602322
Coord A exon
chr2:24602146-24602226
Coord C2 exon
chr2:24602011-24602145
Length
81 bp
Sequences
Splice sites
5' ss Seq
GCTGTAAGT
5' ss Score
8.56
3' ss Seq
CTGCTAATCCTGCTTTACAGGAG
3' ss Score
7.7
Exon sequences
Seq C1 exon
ACTGAGGAGGACCGCAAGAATCTGGCTCGTCTGCAAGATCTGGTTGACAAGCTTCAGCTAAAGGTTAAAGCTTACAAGAGGGCTGCAGAGGAAGCT
Seq A exon
GTAAGTATTGGCCAAAATATCACACATTTTTGCTAATAAATTGTACTCTACAGTCTCAGAACTGCTAATCCTGCTTTACAG
Seq C2 exon
GAGGAACAAGCCAACACTAATCTTAGCAAGTTCCGGAAAATCCAGCATGAGCTTGATGAGGCAGAAGAAAGAGCTGACATTGCAGAGTCACAAGTCAATAAGCTACGTGCCAAAAGTCGTGATGTCAGTTCTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:37
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.312 A=NA C2=0.956
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(3.6=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=PD(4.7=88.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGCTCGTCTGCAAGATCTG
R:
TGTGACTCTGCAATGTCAGCT
Band lengths:
167-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]