DreINT0177874 @ danRer10
Intron Retention
Gene
ENSDARG00000077987 | zfat
Description
zinc finger and AT hook domain containing [Source:ZFIN;Acc:ZDB-GENE-130204-2]
Coordinates
chr16:25432658-25434442:+
Coord C1 exon
chr16:25432658-25433916
Coord A exon
chr16:25433917-25434209
Coord C2 exon
chr16:25434210-25434442
Length
293 bp
Sequences
Splice sites
5' ss Seq
GTGGTATGG
5' ss Score
5.61
3' ss Seq
CTGTCATGTTCCCTCAACAGGTA
3' ss Score
12.03
Exon sequences
Seq C1 exon
GTTAGGACAGACACAGCTTAAAATCTTCACTTGTGAATACTGCAATAAAGTCTTCAAGTTCAAACATTCACTGCAGGCTCACTTAAGAATCCACACCAACGAGAAACCCTTTAAGTGCATGCAGTGTGACTACGCCAGCGCCATAAAGGCTAATCTTAGTGTTCACATGCGCAAACACACCGGAGAGAAGTTCAGCTGCGACCTCTGCTCGTTTAACTGCCTCAGTAGAGGACACCTCAAAGTGCACGTAGAGCGAGTGCACAAGAAGATCAAGCAGCACTGCCGCTTCTGCAAGAAGAAATACTCAGACGTCAAAAACCTTCTGAAACACATCCGCGAGTCTCACGATATGAGCGACAAGAAAGTGCAGGAATCCTACGAAGAATACAGCCTTCAGACCAGGGAAGGCAAGAGGCAGCTTCTGTACAACTGTGAAATATGTGACCGGAAGTTTAAGAACGAGCTGGAGCGTGACCGTCACATGATGGTCCACGGCAGCAAGAGACCCTTCGGCTGCGAGTTATGCGATCACGGCTCCACAAAATACCAAGCCTTGCAAGCCCACATCCGAAAGCATCCCTTCATCTATGTGTGCGCCATTTGCCAGGAAAAGTTTGTCAGCTCTGTTCGGCTGAGGTTGCATCTCCGAGAGCTCCATCAGGAGTTGGATGAGCTTTCAGCGTTTGCAGACTCCATCAAATCCAGCTTTTGCTTGCTTAAGCCAGGTGATGACATTCAAAAGGACATGTTGAGTCAGGATGAGCTTAAAATCACAGAGGAGCTGTCGCAGCTCAATGCCCAGGAGCTGTTGACCACTGAAATGAGCTGTATGGCTCAAGAAGACCTTCAGTCTCAGTGCTCGGAGAGTTTAGTGGTGGAGTCGGACCTTCTCGCAGAGAACTCGCAAGAGATGGATACATGTGCAGTTGCGCAAACGCAAGAGATGGCCTGTGAAGATGCCATAAATACGCAAATAACTCAAGATGAACCACTTGAAGTGCAGAGCAGCGTGACAAAAGAAGAACAGACTCCTGTTCAGGAAACTGTTGCTGAAAATGCACAAACAGACCCAGAGCAGCTCCCAGAGGAGCATCAAAATGAGCTAATTACACAAGCTAATGCAGAACTACACATTTCAGAGCAAGAGGAAATATCTGCTTTCAAACAGATCCTAGAACAGATGCAGAAACGACAGCTTAACATGGAAGTGTTTGAACGGATTCGGAAAGTGTACGGAGATCTTGAATGTGAATACTGTG
Seq A exon
GTATGGATATCTCATACATTACATAAAATATTTATATATAATAATAGTATCATAGTCTCTATGTAAGACACTGTCCATAAAATAGTAATGAAGATCCACTGAGATAATTTCAGCATCTTTCAAAAAGACATTTGTGTCATTGTTTACTCCAAACCAGTATGAGATTCTTTCTTTCATTAACCTCAAAATAAGATGTTTCCGAAATGTTGAAAACTGATTCAATAGCCAAAGATGCTGGCGTTATATTAAATCACCAACTAACTCTTTAATTCTCTGTCATGTTCCCTCAACAG
Seq C2 exon
GTAAGCTTTTCTGGTACCAAGTGCACTACAATACACATGTGCGAACACACACGAGAGAGCACCTCCATTACTGTTCTCAGTGCAGTTATTCCTCCATCACGAAAAACTGCCTGAAGCGGCACGTCATTCAGAGGCATTCAGACATCCTGCTGAAGTGTCCCTCTGAAGGATGCCAATACTGCACACCTGATAAATACAAGCTTCAGGCTCATCTCAAAACCCACTCGGACATA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000077987:ENSDART00000156351:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.172 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF134651=zf-H2C2_2=WD(100=5.9),PF134651=zf-H2C2_2=WD(100=5.9),PF056057=zf-Di19=WD(100=8.1),PF121713=zf-C2H2_jaz=WD(100=5.0)
A:
NA
C2:
PF134651=zf-H2C2_2=WD(100=33.3),PF134651=zf-H2C2_2=PU(44.0=14.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCACTTGAAGTGCAGAGCAG
R:
TGCACTTGGTACCAGAAAAGCT
Band lengths:
297-590
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]