DreINT0177941 @ danRer10
Intron Retention
Gene
ENSDARG00000027109 | zfr
Description
zinc finger RNA binding protein [Source:ZFIN;Acc:ZDB-GENE-030131-378]
Coordinates
chr5:40534716-40536692:-
Coord C1 exon
chr5:40536492-40536692
Coord A exon
chr5:40535005-40536491
Coord C2 exon
chr5:40534716-40535004
Length
1487 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAT
5' ss Score
7.87
3' ss Seq
TTTAATATTTTTTTCAATAGGTT
3' ss Score
10.05
Exon sequences
Seq C1 exon
ACCTATAAAGAGCATCTCGAGGGTCAGAAGCACAAAAAGAAGGAAGCTGCACTGAAGGTGTCACAGAGCAGCAGTAGCAGCAGTGGAGGCGGGAGCTCTGCCCGTGGGACACAAAATCAGTTGCGCTGCGAGCTCTGCGATGTCTCCTGTACTGGGGCTGATGCCTACGCTGCGCACATCAGAGGAGCCAAACACCAGAAG
Seq A exon
GTACATGACTCACTCTCTGATCTCCGCACATAATAACCTGCTTTAAGCTTGTTTTGGCAGCACTATTTTTGAATGCGTATAATTTTGGATCAGTGTTGTGGAGAGGGTTTCTTAGGTCTTGTCTACCAAAGCATTTTTGTGAGAAGAAACTCCAGTGCTCTTCTAAAATGCTTAGCTGTGAGTGCTTCAACTGTCAGACCATTAAAAAATCTTGGTGCCTTGCAACACTGCTGTTGTTTAAGAAATGTGGCTTTTTCCATTGAAAATAACTGGAAAGTATACATGTTCAGCTCTAAATTTACATTTTGGTGAACAAATGTCCTTAGGGACTCTATTACAAATATGAATAAAATTTGTGTGAAGGGGATTGAAGGTACTTTATTCAGGGATTTGATACTTCCATATAAACGCTGATTTCTGTATTTTCAGAGTTCAATGGGGTAACCAATGTATTTTGCATAAATCCGACGTCTTTCAGGGTCAGGGATGTAATTGTAGGCCTACCTAGGCTACTGTGTTTATAACAATGACATTGTGTTGTTCTATAATTAGCACATGACAAAGCTGCTGACTATGCAAAAATTATGAAATCACTTTGAATGGGTGCGTGGCTGTGCACACCGTTTCAGCTCTCCCATTTGCACATCATGACCACGTGCACTCTATATACAATAAAAATAAAGCCAGGATGCACATTTGTAATCCTCTGCAAGTCATCTGTTCTTATGTCTTAATTTATTCAGTGCTAGAGCCTATAGCACCTAAAAAGTCATGACAGAAAGTTATAAGTTTAAATGATTTTAAAAAATTTATAATTAAATATTTTTAATTTCAATTATTTCTTTTTTTTCAGGGTGCAATGTTAAAGTATATTAGGCTACTCTATTCATCTGGACTAATTATTTTTACAGTTCGAATTGATGACTGACTCGAGTTATAGCCTTATAACATGTGAAATATAGAGTTGACTAAATTAGAGGAAAAATTCTATCCTGTGGACACGAGAACTAACAGTTCGTGGCGCTTTCTAGCGGCAGTGTATGGATCTGTGTCTACATTGAAGTACAAACTATATACCTAAATATTACTCGAGTGTCTATTAAACTGATAAACGTTTAAACTGATACAACAATCCTGAAACACAAATTGTATGGCCAATATCAAATATTTATATCAAGAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAATGTGTGCGTGTTTGTAATTATTATTATTTTTATTTTTTATTTATGTATTTTTAGTTTTTGTCATTTTTATTTTATTTTTTGTCCTTGGCTGATTACATGCCTGTTTATCACAAGCATATTATATTAGTTGTCTAATGCAAATCAAAGTGTGTTTGTAGCATTGAATTCTTTAGTTGGTGGTGTGTTTATTTTTTTCTTTATATATAAATTATTCATTTAATATTTTTTTCAATAG
Seq C2 exon
GTTGTAAAATTGCATACCAAGCTAGGAAAGCCGATCCCATCCACTGAGCCCAGTGTGGTTTCCCAATCTTCCACCTCCAGTACTGCAGCTCCGAATAAAACTTCCAGCTCCAACATCAACAGCTCCACAAGCACATCATCAAATGCTTCTTCCTCTGTTTCTGCCTCTTACTTAAAATCTGGCAGTATGGCTGCTTCTGCTGCACAGGGCAAGAGTTCAGGAGCCAACAGTGTCTCTGTCAACAGCTCCTCTATCAAGAAGGTCAACACACCCAAGATCAACTTCGTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000027109:ENSDART00000074789:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.582 A=NA C2=0.856
Domain overlap (PFAM):
C1:
PF128742=zf-met=PD(40.0=14.9),PF128742=zf-met=WD(100=37.3)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCACAGAGCAGCAGTAGCAG
R:
GCAGCAGAAGCAGCCATACTG
Band lengths:
345-1832
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]