GgaINT0073926 @ galGal4
Intron Retention
Gene
ENSGALG00000011982 | PDE5A
Description
phosphodiesterase 5A, cGMP-specific [Source:HGNC Symbol;Acc:HGNC:8784]
Coordinates
chr4:54098790-54100826:+
Coord C1 exon
chr4:54098790-54098965
Coord A exon
chr4:54098966-54100766
Coord C2 exon
chr4:54100767-54100826
Length
1801 bp
Sequences
Splice sites
5' ss Seq
GAGGTGAGC
5' ss Score
8.7
3' ss Seq
AGGGCTCCTTTTCTCTTTAGGTT
3' ss Score
10.31
Exon sequences
Seq C1 exon
GGGTTTGCCAGCTTGTGAATAAGATGGAAGAAAACTCAGGCAAGATCAAGGCTTTCAACAGAAATGATGAGCAGTTTCTGGAAGCATTTGTCATCTTTTGTGGCTTGGGGATCCAGAATACACAGATGTATGAAGCTGTAGAAAGAGCAATGGCCAAACAGATGGTGACATTAGAG
Seq A exon
GTGAGCTCAAAAACTAGGTGATGTTTGATTGGCACAAAACAGATATTCATATTCTCCAAAACTTGCCTGTTCTACACATAAATGCAACTATTTTCACATGAACAGAAATTGTTCTCCCTCATGAGAACAGCATTTGGCCTGATGAGTTGCACAGGAATTTTTCTGCTTGTTTCCATGTCTCCCAGCAGACTATTTTATTTGGTAGTGTATTTTAACTTGACTCAGGAGACTTAGCAGTGAACTGGACACATCTTTTCCTTTATGCAATGCTACACATTGGCAACATAGTTAGTTTGTTTTGTTGCGTAGGAAGAGGAAATGGTTTAGGGGATTAGGGGCTTCCTTTTCTTGTTTGAGGTTTTGTGTGATGATTTTGGCTCAGTCCTTTAGACAAACTGGTTTGTGGAGCAACAACAGCATTATTGTAGGTTTTCAGCAATTTCCTCAAACTCCATCAAACCATATCAAGATGAACGCCATCTAGTTTCAGCCATTGAATTGGTTACTGTTAAGTTTAATAGCTAAAGAGGGCTTTAGTTAGAGAAGCTGAATCACTCAGAGGTGATAACTTGAAGCTGAATGTGAAAAATTTGTAGACTTTGGATTTCCTAACAGTCTTTATGTAAATAGAGTATTTGGAGGATTTTAAAATGTGGATTATGTACCCTCTAGCCTATAAAAACAGGAAAAAAAAAAAAAAAAAAAAAAAAAACCATCACCACTCTCAAATAAACCTGTATAAAATGACATTAGATAAAAAAGTAGTAAAACTGTTGTCAAAATAATTTAAGTACTTCAAAAAGAAGCTGTTTGGTTACATGGCAAATTAGCAATTCTTTCTCTTACCATAACGCTTTACAATATATGAAGTCCATACCTATAACTTCCATAAGAAGATTATAAGTTAATACCATGTATGGGAACTGTTGAATCACAGCTTGAACCTTGGATGGACCACCTGAGGCAAGCACTGAGTCACCGTGGGAGCACAGGTGAAGGCAATTCAGCTGTGTGACCGGAGGGGGTGGAGCGTGGCTGCACCTCTCCTAGACCCCATGTAAGGGCTGACTGCCACTGAGGAAGGATCTTTCTCTGGAGATCATTCTCTTTGGAGTTTTTCTGCAAGCCTACAACATGGATGAGCATTTCATTTATTTCTGATTATTGCTTCCCACTGTGATAATCTTTCTAGCTATACAATCTGTAGATACCAGCCTAACCATGCCACTTTGTGTATTTGTTGATTGTACACACCTAAATTGACTTCTTTATAAAATATTTTTTCCTTTAGTTGTTAAAAAAGTCTGAAACATTTTCATGTTCTGAGTTCTCACAACTGAAAAAATATGAGCATATTTTGTTAATTTAAAACACAGATGTTTTCTTTTAGACTTTCCTGCGGAATTTCAGTTAAGGCAAAATTTTCTTTTATGTCTGAATAAACTCCTGAAAGGATTATTATGGCAACAACGTCAGGCACATAATTATAGCTCTTTCATGAATTATTTGTCTTTGAAAGTGGGGCATGAAAACTTTCCAAAGAGAAAATCAGAGTTCTTTGCTCATTTATAGAAACTATTTACGTTTTCTTGGCCTAATTATATCAAGAAGATTCTGTGCTATGGCAGATCTCTCCCCACATGCTTTCAGAACTTAAAAGCAGCGCAGGCCCTGCAGGGACTCCACATTTCATCAGCAAGAGCAATGTAATGCAGCTGCATACTGAAAATTCTGCTTGCTGAAACCCTTTGTTTTATCACATTGCTCTTTTGATTGTACTAAGGGCTCCTTTTCTCTTTAG
Seq C2 exon
GTTCTCTCATACCATGCTTCTGCTGCTGAGGAGGAATCAAGGGAGCTGCAGGTAACAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000011982:ENSGALT00000019550:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=PD(23.6=62.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTTTGCCAGCTTGTGAATA
R:
CTGTTACCTGCAGCTCCCTTG
Band lengths:
234-2035
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]