RnoINT0108873 @ rn6
Intron Retention
Gene
ENSRNOG00000014443 | Pde5a
Description
phosphodiesterase 5A [Source:RGD Symbol;Acc:620995]
Coordinates
chr2:226982479-226984181:+
Coord C1 exon
chr2:226982479-226982654
Coord A exon
chr2:226982655-226984121
Coord C2 exon
chr2:226984122-226984181
Length
1467 bp
Sequences
Splice sites
5' ss Seq
GAGGTGGGA
5' ss Score
4.3
3' ss Seq
GGTTCCTGTCTTACCCATAGGTC
3' ss Score
10.35
Exon sequences
Seq C1 exon
GGGTTTGCCAACTTGTTAATAAGATGGAGGAGAAGACTGGCAAGATTAAGGCTTTCAACCAAAACGATGAACAATTCCTGGAGGCTTTTGTCATCTTCTGTGGCTTGGGGATCCAGAACACACAGATGTACGAAGCGGTGGAGAGAGCCATGGCCAAGCAGATGGTGACGTTAGAG
Seq A exon
GTGGGAAGAAGCCCGTGGGATTTGGTCTGCCCGGGGACACACTCAGCTTCTCCTCCTAACACTCACAGCATAAAAACACGGGATGCTCATGATGATGCCCATATGCTTGTTTAAAAGCTAAAGAAAGGAGATCCCAAATATTTAGACTCTTACCAAGGCTAATAGGACCACACCAAGACTCTGGTCCATCTCCTGCTTTTTCTGCTTAAAGCAGTATTTGCCTGCTGCTCTCCATTCATGGGGATACCCACCATCACTGTGTCTGTCTCTTCCCTGTTTATCTCCCGCTTGCACATCTCTTTATGAGGATATCTATTTAAAAACAAAAACAAAAAGCTTTTTAAAAAAAATCCCCTACTGGGCTGGAGAGATGGCTCAGCGGTTAAGAGGACTGACTGCTCTTCCGGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCCTCTGTAACGGGATCAGATCCCTTCTTCTGGTGTGTCTGAAGACAATTACAATAATATATAATAAATAAATCTTTAAAAAAAAGTCCCCTACCAATCTGGGAGAATTGAAAGTAAGGAAAAGAAAAAAAATAAAGGAAAATAAATAAAGGAAAATCAGTAAATGGCAGAAACAAGTGTTTCACACTAACGGGTCAGGGACTTTTCCTTCTGATACAGCTAGGTTTCTGGAGAGGCTGCAAATATTATTCCCTCTGTGAACTAGAACTTTACATTTTTTATTTAAGTATAAAATAAGCCTTTGAATACAGCCACATCTTTGGTTTAAAGGGAATTTCTAAAATTATCCCCCATTGCTTACAAGTCACCCTTTTTGAAAATGAGAATTATGATTCTTATCTCGTTCGTTAGAAGGTTTAAAGTAAGCCCAGCTCGTGAAGAAGCACTGAGTCCTGGTTCTCCCGTGAATAGCCAGTGTCTTCAGCTGCCGCTTTACTTTCTCTGTCCCTGGCTTGTAGACTCTAAGAGCCCCTTTCATCCTGCGTGAGTGTCCATTCCAGAGTGAATTTGATAGTTGTTACAGATTTCACCTGAGAGCCATTTCTAAACCACTTCCCCCCATCCCAACAGCAACTCTTCGGGCAAGAGTTTTGCACCCCTAGGCTGAACACAACAAGTCTGTTTCTGAAAAAAAAAAAAAAAAACAGTTCAAAATAATCTTGCTGCTGCGTGAGCCAGTCATCCTTTGTAGAGTGTCTTATAAACAATGGGTTTGTTATATCAACAGTGTCAGAGTGAAAGTTTCAGGACTTTCTTTGAGAACTAAGGAGTGTAGCTTCAGAAGCACACAATGAACCCTCGATGGAGGTTTCACTGTGTTGATGAAAGCCACAACTGAGAGTTGGCAGTGAGAGACCCTTGTTACCTGCCTCACAAGTTGGAGCAGGACTCCAGTCGGATGTTCTGTGTGCATCAGTCGTCCTACCTGTGTAACCTGGGTTCCTGTCTTACCCATAG
Seq C2 exon
GTCCTGTCTTATCACGCATCAGCGGCGGAGGAAGAAACAAGGGAGCTCCAGGCTCTAGCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014443:ENSRNOT00000019638:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=PD(23.4=62.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTTTGCCAACTTGTTAATAAGA
R:
GCTCCCTTGTTTCTTCCTCCG
Band lengths:
222-1689
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]