GgaINT0119203 @ galGal4
Intron Retention
Gene
ENSGALG00000015358 | myosin
Description
myosin heavy chain, cardiac muscle isoform [Source:RefSeq peptide;Acc:NP_990097]
Coordinates
chr1:86887019-86889098:-
Coord C1 exon
chr1:86888983-86889098
Coord A exon
chr1:86887212-86888982
Coord C2 exon
chr1:86887019-86887211
Length
1771 bp
Sequences
Splice sites
5' ss Seq
AAGGTACTG
5' ss Score
8.56
3' ss Seq
CTACTTTATCCAAAAATCAGGAG
3' ss Score
2.5
Exon sequences
Seq C1 exon
GGAGCTTTGGAGCATGAAGAAAGCAAGACCCTTCGTTTTCAGCTTGAGCTTTCTCAGCTTAAAGCTGATTTTGAAAGGAAGCTGGCAGAAAAGGATGAAGAAATGGAAAATATAAG
Seq A exon
GTACTGTGCAGAGCTTTTATTTACGTTTCTTCTGCTAATAAAAGGATCAGGAATATTCTCAAGACCTAAGCCAGCCAAACAAACCACAGCTAGTTATGCCAGCAAAAGCAGATGCTGGCAGTACCAGTAACTAAGGCAGCTCTGGTTATTGCCCTCTGCCAGAGGGTAGTTCTACCACCAGATCTTTTCTTAGTTCAGATATTCCTGTCAGTCCAGTCTCTCCAGTGTACATGTAAACCCGCCAGACACATCCCAAAGCAGCTACAGGAAAACCAGCTGGCACAGTCATGAGCTGCTAAGCCTGTATGTTTACAGTAGGAAACAAGCAGACATGCAAAAGACAGTTTGCTGCCTCCTTGCAGTCTCCTAGAATGAGCCCCTCCCAAGGGGAACCACCTGGGAGTGGGAATCAGCAGAACTGTTGCAGTGGTGCTCAGATTGATGCTACCTGAAGGCAACATTTAATTCTGCATCTGGTTCATTTGTGCAGTCATAGCTACACTGTTTGCAGGAGGACACAAGGAGCTTGCAGTTAGGCATGCACAATTTGGGTAAGCGGTGAGATTTGATGAGGTACGGAAAGCGTGGATATAAAAGGAGAGGAACCTGCAGGTAAAAAGGGGGTGAGCAGCAGGAAAAAGGAAAAATAATCAGATGACAGAAGGATGAAATCTTTTCCCCTCTTTCCTCTAGCACAAACCCTAGTCTGAAGAGTTAGACTGATTACAGAGACCACAGGACAAGCCATTCCATTCGGTGTTCCTGACTCAGCCACACTGCGTGTTTTGCTGCTGGGCTGCCCAGCAGGCAGGCAGTGCTATCCTTGGGAATCTGGGGCACAGTTCTCTGTCTTGCCCAAGCAATAAGACTGTCAAGGTTTTTCTTGTTGCCTTAATGAGTTCCCAGAAATGATATGCAAACAGCAAAGCCCGGCCAAGGGTGAGATGAAATTTTTTTACTGTGTGAGTCATACTCTGTGCATTGAGTTACTTTCTGGCTGCAGGAGAGCAAAACCTTCACCTTGGAACTCAGAGCGTAGAACTGCGGATTTTAAGCCTCTCTGTTCTGACTGTTAGAGGAAAATTATGCACCTGTCTCTATTTGTTTTGCCATCTCTTAAATTGACAGTAAGCTGCCTTTAACAGGGGGACCAGCCTGTTAATTACAGTGGGCTTAATCTCTTCTAACTTTATACGTCCATAACACAGATTTCTATTCCCAATCTAGTCCCATGATAATTCAGCATACAAAATATATGTTCCAACATCTACTTTTAGGTGCACTAAATTGTAACTTGCAAGTGCCAATTATTATATAAAGAGCCCCTAGAGTGATTAGCTTGAATGTAGCTACCAACCGTCAGTTTGATTAATCTCCTTTCTTAATGCCTTCATGTAAAGCATCCCAGTAGATAATTCAGTAAATATGGCAAGTGACAGTTAAGCATGAAGTGCTTCTTGCGAGGTTCAGGCCTGTACCTGCTTGTGCTCATAAGATACTTGAGGAAGCTCCCTGCTGAAAGACTGCCAAAGAAAACACGGGAGAAGGAAGCTATAGGTGCTTACAGATCAGGAGTTGGCTGAGAGTGTGAAACACAGCAACAAGGTGCTAGAAACCCATGCTATGTGTGTCACTCAGAAATGTAGTGCTGCTTCTAGATGAGACACCTCTTGAAAGTGAAATACGGTTTCACGTAGATCTACGTCCCTTTCCGATGAGTAAAACAACTCCCTTCACCCTTAATTTCCCATCTACTTTATCCAAAAATCAG
Seq C2 exon
GAGGAACCAACAACGCACCATAGATTCTCTGCAGTCCACCCTTGATTCTGAAGCCCGGAGCAGAAATGAGGCCATCCGGCTGAAGAAGAAGATGGAAGGAGACCTCAACGAGATGGAAATCCAGCTCAGCCATGCTAACAGGCATGCTGCAGAAGCAACCAAGTCAGCACGTGGCCTGCAGACACAAATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000015358:ENSGALT00000036887:34
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.590 A=NA C2=0.846
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.4=100),PF0003816=Filament=PD(10.1=79.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAGCATGAAGAAAGCAAGACC
R:
CTTGATTTGTGTCTGCAGGCC
Band lengths:
302-2073
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]