GgaINT0129029 @ galGal4
Intron Retention
Gene
ENSGALG00000000779 | MYH13
Description
myosin, heavy chain 13, skeletal muscle [Source:HGNC Symbol;Acc:HGNC:7571]
Coordinates
chr18:316125-318015:-
Coord C1 exon
chr18:317879-318015
Coord A exon
chr18:316381-317878
Coord C2 exon
chr18:316125-316380
Length
1498 bp
Sequences
Splice sites
5' ss Seq
AAGGTAATA
5' ss Score
8.49
3' ss Seq
TTATTTTCATGCATTTTCAGAGA
3' ss Score
8.9
Exon sequences
Seq C1 exon
GTGTTCTTCAAGGCAGGTCTCCTGGGACTCCTAGAAGAAATGAGAGATGAGAAGCTTGTGAACCTCATCACTCATATGCAAGCTATGTGTAGAGGGTATCTCATGAGAACTGAATTTAAAAAGATGAATGCAAGAAG
Seq A exon
GTAATACATGAACACAGAACAAGTCATATTGTGCCAGACCAAAAGTCCGGGATTGCAGCATCCCATTTTAGACACAAATCTGTCCTCAGTGAAGACATTGAGAAAGATTAGGGGAAACAGAAAAAGCAGAAACTAATACTTTCCTTGTTGTAGCATTCCAAATTCTGGCAATGGCAGTTTAGTAGTTGGACTAAGCATTTGGACCATGATACTTAACAGTGAGCCCTTCCTCCAGGAATGTAATAACCTTTAGAATCATTTTCAACCTTTAAAGAACCCTGTGATGGAGATAGACAAACCTTAGTACTTGTTCATTTGGCTGTTTGGAACATGCTGTCTGACAATTTCATTAAATCACATCAGTTTATAACGAATGGATTCACATTCTACTCCTGTTTTATTTCATTGCTTCATTTCTGTCTAAGATGATGTATTGCAACTTGTACGGAGAAGTTATGTTATGTGGAACAGTTGAGAAAATACACTTTTAATTTCTGTAATTTTGAGTGGAACAATTCATGCAAAATTTGGAGGGTAGGGGTGACAGTACTGCACACACCAAGCAAATTGTTTCAAATATTTTTGACAGGTGAAAGCTGTTTAGGGGGAGCTAGGGCGACATAAACCCACATAAACTGCCAACTTGTAGCCTTAATCTGTAATACCCTAGCATATATGTTTAACTCAGCAGCTTCCCAAAGCAGGGGTAGTTTCTAACAATTCGACTACCTGTACTGGATTTCTCTGCAATGAACTGTCAAAGCTGCCTTGAACAAAATCCTTTGGCATTCAGTAGAAGCAATGAACATCTGGAGGGGACCCACAAGAATCACTGAGTCCAACCCCTGGCTCCATATCCTGCTCCTCCTGCTGCAACCCTGTTCCAGTGACCTCTGTGCCATGGAAGACACTGCTGGCACTGCAGGTGGAGCACAGCACGCTGGGCCCATGGTGGTTCCTTGTGCCCTGTCCTTAGGGTCGTGGTTAGAGAGCCAGAGCTAGGACTGTTCTGCAGCTAGGGGCTGGAGCTGCCATAGTAAGATTTCATTGTTGTTTTCATTTTGCATCTTAGTAATTTCTAATATTATAGTTGTTGTTTTTTCGGACCCAAAGGCATCAAGCTGCTGTTTCCACAATTTACAGTAACACCCATGTTTATAATCTCAACATGAATGTCAGTTTTCACTCTGGTCCTCACCCGAGCACTTGCACTTTGATATTCAGGAAAGTTTCAGAGATTATCAGACAACACAACAGAAAATTCTAGCTATTTTGAAGTCCTCCCTATTATGCCCAGACGTAGCTATTGTTCGTTATTGCCCAGACTGAGCCAGACATTCAGCAGTCTGGTGCCTAGGAATGACTTTAATAAGTACAAGTAAGGTGAGATAAAACTTTCCTGAACAAAATGCTGATACTGTGTTACCAATAACAAGAGTCTGTTTCCAACCCAAGTCTCTCCAGTCTCATAACCTATATTATTTTCATGCATTTTCAG
Seq C2 exon
AGAATCCATTTATATAATCCAATACAATGTTAGAGCATTCATGAATGTCAAGCACTGGCCCTGGATGAAGCTGTACTTCAAAATGAAGCCCATGTTGAAGAGTGTAGAATCTGAGAAGGAGATGGCCAACATGAAGGAGGAGTTTGAGAAAACCAAGGAAGAGCTTGCAAAGTCTGAGGCAAAGAGGAAGGAGCTGGAGGAGAAAATGGTGACCCTGTTGCAAGAGAAAAACGACTTGCAGCTGCAGGTTCAGTCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000000779:ENSGALT00000001184:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.326
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.4=6.5),PF0061222=IQ=WD(100=43.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTCTCCTGGGACTCCTAGA
R:
CTCTTGCAACAGGGTCACCAT
Band lengths:
349-1847
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]