GgaINT0143532 @ galGal4
Intron Retention
Gene
ENSGALG00000000410 | TLK2
Description
tousled-like kinase 2 [Source:HGNC Symbol;Acc:HGNC:11842]
Coordinates
chr27:2394844-2396080:+
Coord C1 exon
chr27:2394844-2394955
Coord A exon
chr27:2394956-2395972
Coord C2 exon
chr27:2395973-2396080
Length
1017 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGA
5' ss Score
10.57
3' ss Seq
TTTTGGGTTTTTTTTGGTAGCCC
3' ss Score
5.36
Exon sequences
Seq C1 exon
GTATTTGCCACCAGAATGTTTCGTGGTTGGGAAAGAACCTCCAAAGATTTCAAATAAAGTTGATGTCTGGTCAGTAGGGGTCATCTTCTATCAGTGTCTCTATGGCAGAAAG
Seq A exon
GTAAGAGAGCATGTAACTCCTTTCCAAGCAGTGATTCCCAAACCTATGGGAGGCAGCCCTGGCTTCTGCTCCCTTCTCCTTGCCAGACTAGAAGGTGCTTCTGGCTTGTCTCCTCGCTACCAGTTCCAAGACAGAGTCCATGTTAATTGAGTGAGGATAATTTGTTAGGTTGTGTTTTGCTCTATATGGCAATACTTTCAGAAGCAACATTCTTCAGAACATCTTTCCTGTTGGTTGTATATCCACTGAGATGATGATGGGCAAGATGGATGTGTCATTCAGGACCACGCACCTGACACTCTTGCTTTGGCATTGTTTTATCATGCTTTCAGTGCTCTGATGTTATTCTTTGAGTGCAAAGTCTTTGTCAGGCCTAATATTGCTCAACAGCTGCAAGTGTTTTAAAATAAAAAGCAGATATAAAGACCTACTCTAAATGTTTTTAGAATGCCTTTTGAAAGGGAAGAGTCTATTTCAGGAAGAGGTGATTTGTTCTGAGAAAAGGCAATCTTACTTTTTTTTTTTATGACAACAGAAGAAGTTGGTTGGGCAGCAAAAGGATTCCATCATCTCTTATTTTTACAAGTCTTTGGTAACCAGGAATTGGGAAAGCCTGGGCTGATAGAAACAGTGTGGTGTCAATCAGGCAAACAGTGGAACCAGCCCAGCAGGTTGAAGGAGGTGAATCTCCTGGCAGCTCAGATGGGTGAAGGTCTTCTTGCATGTGGTCAAGCTGACCTGTGAGGTCTGCAAGCACATGGAGTTCCTTCCTGTCTGAGCTGGAGACACACCGGACATGTTCCAGCTTGGTGTCCCTTTTGTTCAGTTCAAAAACAGAGCAGTGTGTCAGATGTTCATCTGGCTGGAATCTGCAAGCACCTTCGCAGTGTTCATGGGCTTCTTGCCTGGAAAAGTGCTGATCTCAAGCGCTGATTAACAGTAGCACTTTGGGATTATTTTTTTTTAAGGTCTGTCATCAGTCTTTAACTACTATTGTTTTTGGGTTTTTTTTGGTAG
Seq C2 exon
CCCTTTGGCCACAACCAGTCACAACAAGATATTCTACAGGAGAACACGATTCTGAAAGCTACCGAGGTGCAGTTCCCTCCAAAGCCAGTAGTCACACCAGAAGCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000000410:ENSGALT00000000559:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(13.2=100)
A:
NA
C2:
PF0006920=Pkinase=FE(12.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTTGCCACCAGAATGTTTCGTG
R:
GCTTCTGGTGTGACTACTGGC
Band lengths:
214-1231
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]