HsaEX0014018 @ hg19
Exon Skipping
Gene
ENSG00000158402 | CDC25C
Description
cell division cycle 25 homolog C (S. pombe) [Source:HGNC Symbol;Acc:1727]
Coordinates
chr5:137627659-137664218:-
Coord C1 exon
chr5:137664173-137664218
Coord A exon
chr5:137654908-137655063
Coord C2 exon
chr5:137627659-137627805
Length
156 bp
Sequences
Splice sites
3' ss Seq
GTTGTCAATATACTTGACAGGAC
3' ss Score
3.98
5' ss Seq
AAGGTAAAG
5' ss Score
9.06
Exon sequences
Seq C1 exon
GTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGAT
Seq A exon
GACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAG
Seq C2 exon
GTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000158402_MULTIEX2-3/3=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.632 A=0.615 C2=0.523
Domain overlap (PFAM):
C1:
PF066178=M-inducer_phosp=FE(23.1=100)
A:
PF066178=M-inducer_phosp=PD(10.5=7.7),PF066178=M-inducer_phosp=PU(42.9=98.1)
C2:
PF066178=M-inducer_phosp=PU(71.6=98.0)
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCACCTGGATTCTTCAGGACT
R:
CTTCCTGAGCTTTCCTTGGCC
Band lengths:
193-349
Functional annotations
There are 5 annotated functions for this event
PMID: 11842186
This event
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: competition binding, mutation analysis. ELM ID: ELMI001012; ELM sequence: NKEND; Overlap: PARTIAL_LEFT
PMID: 11897663
This event
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, inhibitor. ELM ID: ELMI002160; ELM sequence: EISDELMEFSLKDQE; Overlap: FULL
PMID: 11897663
This event
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, protein kinase assay. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 12738781
This event
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, electrophoretic mobility shift assay, protein kinase assay, western blot. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 14968113
This event
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, mutation analysis, protein kinase assay. ELM ID: ELMI003380; ELM sequence: EEISDEL; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Autistic and control brains
- Pre-implantation embryo development
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)