MmuEX0010364 @ mm10
Exon Skipping
Gene
ENSMUSG00000044201 | Cdc25c
Description
cell division cycle 25C [Source:MGI Symbol;Acc:MGI:88350]
Coordinates
chr18:34738216-34747313:-
Coord C1 exon
chr18:34747224-34747313
Coord A exon
chr18:34743938-34744063
Coord C2 exon
chr18:34738216-34738278
Length
126 bp
Sequences
Splice sites
3' ss Seq
CTTGTCAGTATACTTGACAGGAA
3' ss Score
2.16
5' ss Seq
AAGGTTCAG
5' ss Score
2.52
Exon sequences
Seq C1 exon
GTACAGCTTCTTTGTAGTACTCCGAATGCTATGAATCATGGCCACAGAAAGAAGATTGCAAAGCGTAGCACATCTGCACATAAAGAAAAT
Seq A exon
GAAATGAAGCATCTGGGCAGTCCCATTACTACGGTTCCTAAGCTGAGTCAAAATGTAAAGCTAGAAGACCAAGAAAGGATTTCAGAAGACCCAATGGAGTGTTCCCTGGGAGACCAGGACGCCAAG
Seq C2 exon
GTGTGTGTGCTGCCAACGGTGCCAGGGAAACACCCGGATCTGAAGTATATCAGCCCAGACACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000044201_MULTIEX1-2/3=C1-C2
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.767 A=0.619 C2=0.000
Domain overlap (PFAM):
C1:
PF066178=M-inducer_phosp=FE(17.9=100)
A:
PF066178=M-inducer_phosp=FE(25.3=100)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGCTTCTTTGTAGTACTCCGA
R:
CTGGGCTGATATACTTCAGATCCG
Band lengths:
146-272
Functional annotations
There are 5 annotated functions for this event
PMID: 11842186
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: competition binding, mutation analysis. ELM ID: ELMI001012; ELM sequence: NKEND; Overlap: PARTIAL_LEFT
PMID: 11897663
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, inhibitor. ELM ID: ELMI002160; ELM sequence: EISDELMEFSLKDQE; Overlap: FULL
PMID: 11897663
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, protein kinase assay. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 12738781
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, electrophoretic mobility shift assay, protein kinase assay, western blot. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 14968113
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, mutation analysis, protein kinase assay. ELM ID: ELMI003380; ELM sequence: EEISDEL; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types