BtaEX6040698 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000005293 | CDC25C
Description
Bos taurus cell division cycle 25 homolog C (S. pombe) (CDC25C), mRNA. [Source:RefSeq mRNA;Acc:NM_001097996]
Coordinates
chr7:51319296-51339707:-
Coord C1 exon
chr7:51339618-51339707
Coord A exon
chr7:51335333-51335491
Coord C2 exon
chr7:51319296-51319448
Length
159 bp
Sequences
Splice sites
3' ss Seq
ATTGTCAATATACTTTGTAGGAC
3' ss Score
5.94
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
Exon sequences
Seq C1 exon
GCACAGCTGTTTTGTAGCACTCCGAATGCCTTGGAGCATGGCCGCAGGAAGAAAGATGCAATATGTGGCTCATCTGCAAATAAGGAAAAT
Seq A exon
GACAATGGAAACTTGGTGGAAAATGAAATGAAACATCTGGGCAGTCCCATTACTACAGTTTCAAAATTACATAAAAATCCAGAGCTAGCAGAAGATCAGGCAGAAGAGATATCAGATGAATTGATGGAGTTTTCACTGGAAGATCAAGAAAAGGCCAAG
Seq C2 exon
CCACCTCTGAACTGGAGCTCCCTGTATCGCTCCTCATCATTGCCAGACAGCTTAAACAGTCCAAGTTTGAAGCAGGTGGTAAAATTCAAGGACAGCACAATACCAGATAAACTAAAAAAGAAGTATTGTTCAAACCAAAAAGAGCTCGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000005293-'12-14,'12-11,14-14=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.600 A=0.925 C2=0.137
Domain overlap (PFAM):
C1:
PF066178=M-inducer_phosp=FE(40.8=100)
A:
PF066178=M-inducer_phosp=PD(5.6=7.5),PF066178=M-inducer_phosp=PU(42.6=98.1)
C2:
PF066178=M-inducer_phosp=FE(41.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACAGCTGTTTTGTAGCACT
R:
TTTCCGAGCTCTTTTTGGTTTGA
Band lengths:
242-401
Functional annotations
There are 5 annotated functions for this event
PMID: 11842186
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: competition binding, mutation analysis. ELM ID: ELMI001012; ELM sequence: NKEND; Overlap: PARTIAL_LEFT
PMID: 11897663
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, inhibitor. ELM ID: ELMI002160; ELM sequence: EISDELMEFSLKDQE; Overlap: FULL
PMID: 11897663
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, protein kinase assay. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 12738781
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, electrophoretic mobility shift assay, protein kinase assay, western blot. ELM ID: ELMI003363; ELM sequence: MEFSLKD; Overlap: FULL
PMID: 14968113
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, coimmunoprecipitation, mutation analysis, protein kinase assay. ELM ID: ELMI003380; ELM sequence: EEISDEL; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]