MmuEX0006892 @ mm10
Exon Skipping
Gene
ENSMUSG00000038023 | Atp6v0a2
Description
ATPase, H+ transporting, lysosomal V0 subunit A2 [Source:MGI Symbol;Acc:MGI:104855]
Coordinates
chr5:124716305-124718558:+
Coord C1 exon
chr5:124716305-124716515
Coord A exon
chr5:124717886-124718005
Coord C2 exon
chr5:124718439-124718558
Length
120 bp
Sequences
Splice sites
3' ss Seq
TTTCTCCCTTGCACCAACAGGCA
3' ss Score
10.43
5' ss Seq
CGGGTGAGT
5' ss Score
9.89
Exon sequences
Seq C1 exon
GCACTTTAGGAAGAAGTTCAACGTCTACCTGGTCTCGGTGCCTGAGATCCTGTTCATGCTCTGCATCTTTGGGTACCTGATTTTCATGATCATTTACAAGTGGCTGGCATACTCGGCAGAGACCTCGAGAGAGGCCCCGAGCATCCTGATCGAGTTCATTAACATGTTCCTGTTCCCAACCAGCAAGACACACGGGCTCTACCCAGGGCAG
Seq A exon
GCACACGTCCAGAGAGTGTTGGTGGCTCTCACGGTGCTGGCTGTCCCCGTGCTCTTCTTAGGAAAGCCGCTTTTTCTGCTGTGGCTGCACAACGGGCGCAATTGCTTTGGCATGAGCCGG
Seq C2 exon
AGCGGTTACACACTCGTGAGGAAGGACAGCGAGGAAGAGGTGTCTCTTCTGGGCAACCAGGACATAGAAGAGGGCAACAGCCGCATGGAAGAAGGCTGCCGAGAAGTGACGTGTGAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000038023-'34-43,'34-41,36-43
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.474
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(8.6=100)
A:
PF0149614=V_ATPase_I=FE(4.8=100)
C2:
PF0149614=V_ATPase_I=FE(4.8=100)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAACGTCTACCTGGTCTCGG
R:
GTTGCCCAGAAGAGACACCTC
Band lengths:
251-371
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types