RnoEX0013273 @ rn6
Exon Skipping
Gene
ENSRNOG00000052704 | Atp6v0a2
Description
ATPase H+ transporting V0 subunit a2 [Source:RGD Symbol;Acc:621006]
Coordinates
chr12:37371619-37374054:-
Coord C1 exon
chr12:37373844-37374054
Coord A exon
chr12:37372183-37372302
Coord C2 exon
chr12:37371619-37371738
Length
120 bp
Sequences
Splice sites
3' ss Seq
CTTTCTCCCGGCACCCACAGGCA
3' ss Score
8.87
5' ss Seq
CGGGTGAGT
5' ss Score
9.89
Exon sequences
Seq C1 exon
GCACTTCAGGAAGAAGTTCAACATCTACCTGGTCTCGGTCCCCGAGATCCTGTTTATGCTCTGCATCTTTGGGTACCTGATTTTCATGATCATCTACAAGTGGCTGGCGTACTCGGCAGAGACCTCCAGAGAGGCCCCCAGCATCCTCATCGAGTTCATTAACATGTTCCTGTTCCCGTCCAGTGAGACACACGGCCTCTACCCGGGGCAG
Seq A exon
GCACACGTCCAGAAGGTGTTGTTGGCTCTCACCGTGCTGGCTGTCCCTGTGCTCTTCTTGGGAAAGCCGCTCTTTCTGCTGTGGCTGCACAATGGGCGCAGTTGCTTCGGCGTGAGCCGG
Seq C2 exon
AGCGGCTACACGCTCGTGAGGAAGGACAGCGAGGAAGAGGTGTCTCTCCTAGGCAGCCAGGACATAGAGGAGGGGAACAACCGCATGGAAGAGGGCTGCCGAGAAATGACCTGTGAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000052704-'51-48,'51-47,52-48
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.044
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(15.8=100)
A:
PF0149614=V_ATPase_I=FE(4.8=100)
C2:
PF0149614=V_ATPase_I=FE(4.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACATCTACCTGGTCTCGGTCC
R:
TATGTCCTGGCTGCCTAGGAG
Band lengths:
257-377
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]