BtaEX6072271 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000007272 | ATP6V0A2
Description
Bos taurus ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2), mRNA. [Source:RefSeq mRNA;Acc:NM_176637]
Coordinates
chr17:54208868-54211755:-
Coord C1 exon
chr17:54211545-54211755
Coord A exon
chr17:54210716-54210835
Coord C2 exon
chr17:54208868-54208987
Length
120 bp
Sequences
Splice sites
3' ss Seq
CTCCCCGTCTGCACCAACAGGAG
3' ss Score
8.19
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
Exon sequences
Seq C1 exon
ACATTTCAGGAAGAAGTTCAATATTTGTCTGGTTTCCATACCAGAACTGCTCTTCATGCTCTGCATCTTTGGATACCTTATATTTATGATCATCTATAAGTGGCTGGTTTATTCAGCTGAAACCTCCAGAACTGCTCCTAGCATTCTGATTGAATTTATTAGCATGTTTTTGTTCCTGGCTAGTGACACAGGTGGTCTTTACCCAGGGCAG
Seq A exon
GAGCATGTCCAGAGGCTGCTGCTGCTCATCACCGTGTTGTCAGTCCCCGTGCTTTTCTTGGGAAAACCACTCTTTCTGCTGTGGCTGCACAGGGGGCGCAGTTGTTTTGGGGTTGGCAGG
Seq C2 exon
AGTGGCTACACACTTGTAAGGAAAGATAGCGAGGAAGAAGTTTCTTTGCTGGGAGGCCAAGACATAGAAGAGGGGAACAACCAAATGGAAGATGGATGTAGAGAAGTGACGTGTGAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000007272-'17-20,'17-19,18-20=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(8.6=100)
A:
PF0149614=V_ATPase_I=FE(4.8=100)
C2:
PF0149614=V_ATPase_I=FE(4.8=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACTGCTCTTCATGCTCTGCA
R:
CCCCTCTTCTATGTCTTGGCC
Band lengths:
242-362
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]