GgaEX0027504 @ galGal4
Exon Skipping
Gene
ENSGALG00000003194 | ATP6V0A2
Description
V-type proton ATPase 116 kDa subunit a isoform 2 [Source:RefSeq peptide;Acc:NP_990054]
Coordinates
chr15:4911978-4913146:-
Coord C1 exon
chr15:4912936-4913146
Coord A exon
chr15:4912338-4912457
Coord C2 exon
chr15:4911978-4912097
Length
120 bp
Sequences
Splice sites
3' ss Seq
TCTTTCCCACCTATGAACAGGTT
3' ss Score
8.2
5' ss Seq
AGGGTAAGT
5' ss Score
10.45
Exon sequences
Seq C1 exon
ACATTTCAAGAAGAAGTACAATATTTATTTGGTTTTTCTTCCTGAACTTTTGTTCATGATGAGCATCTTCGGCTACCTTGTGTTCATGATTTTCTTTAAGTGGTTAGCATACTCTGCAGAGGACTCCACAACTGCTCCAAGCATTCTGATTCAGTTTATTAACATGTTCCTGTTTCCTGGTGGTGAAGCAGATGCCTTTTATACTGGACAG
Seq A exon
GTTGGTCTACAGAGATTTTTACTGAGTGTTGCTTTTCTTTCTGTTCCTGTAATGCTTTTTGGAAAACCACTTTATCTATATTGGTTGCACAGTGGAAGTCGAGGCATTAGAACGTACAGG
Seq C2 exon
CGTGGCTACAAGCTCATTCGGAAAGAGAGTGAAGAAGAACTTTCTCTGCTGCAGCCTCATGATGTGGAGGAAGGAAGCAGTCACTCAGATAGTGGGCACAGAGAAGAGGATGGAGAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003194_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.275
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(8.7=100)
A:
PF0149614=V_ATPase_I=FE(4.9=100)
C2:
PF0149614=V_ATPase_I=FE(4.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCATGATGAGCATCTTCGGCT
R:
AGTGACTGCTTCCTTCCTCCA
Band lengths:
243-363
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]