DreEX6087062 @ danRer10
Exon Skipping
Gene
ENSDARG00000035538 | atp6v0a2a
Description
ATPase, H+ transporting, lysosomal V0 subunit a2a [Source:ZFIN;Acc:ZDB-GENE-091113-4]
Coordinates
chr10:8037300-8041104:-
Coord C1 exon
chr10:8040894-8041104
Coord A exon
chr10:8038948-8039067
Coord C2 exon
chr10:8037300-8037398
Length
120 bp
Sequences
Splice sites
3' ss Seq
TGTTCATGTTCTTGTGACAGATG
3' ss Score
9.2
5' ss Seq
AGGGTGTGT
5' ss Score
4.51
Exon sequences
Seq C1 exon
GCACTTCCGACAGAAGTACAAGATCTATCTCCTTTTTCTTCCTGAGCTGCTCTTTCTGCTTTGCCTCTTTGGGTACTTGGCCTTTATGATCTTTTACAAATGGCTGGCGTACACAGCACGAGACTCACAGCTGGCACCGAGTATCCTCATCCACTTCATCAACATGTTCCTAATGCAGGCCAATGCCACCTCACCTCTTTATCCCGGCCAG
Seq A exon
ATGGGACTGCAGGTGTTTCTGGTGGTGGTGGCGCTGCTGTCGGTGCCAGTGCTGCTGCTTGGCAAACCTCTGTATCTGTACTGGCTGCATCACGGCGGGAAGAGTCTGGGGATCCACAGG
Seq C2 exon
GGCTATGAGCGAGTGCGACGTGTGAGTGAAGAAGATCTGTCCCCATCTGTAGTTCATGATGAAGAGGAAGGACTGAGCGAATCAGGAGGCCCTCAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035538-'18-22,'18-20,19-22=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.030
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(8.7=100)
A:
PF0149614=V_ATPase_I=FE(4.8=100)
C2:
PF0149614=V_ATPase_I=FE(4.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGCCTCTTTGGGTACTTGGC
R:
TCTTGAGGGCCTCCTGATTCG
Band lengths:
249-369
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]