MmuEX0023217 @ mm9
Exon Skipping
Gene
ENSMUSG00000001249 | Hpn
Description
hepsin [Source:MGI Symbol;Acc:MGI:1196620]
Coordinates
chr7:31893984-31895792:-
Coord C1 exon
chr7:31895733-31895792
Coord A exon
chr7:31894110-31894211
Coord C2 exon
chr7:31893984-31894025
Length
102 bp
Sequences
Splice sites
3' ss Seq
CAGCTTGGTGTCTGTTGCAGGTG
3' ss Score
9.47
5' ss Seq
TTGGTGAGA
5' ss Score
6.29
Exon sequences
Seq C1 exon
ATGAGGAACCTGGGGCTCACAGAGGAGGTTCCACTTGTTCAAGACCCCAACCTGGAAAGG
Seq A exon
GTGGCCGGACTGCAGCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCATTGTGGGTACCCTGCTGTTCCTGACAGGCATTGGGGCCGCGTCCTGGGCCATTG
Seq C2 exon
TGACCATCCTACTGCAGAGTGACCAGGAGCCACTGTACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000001249-'6-8,'6-7,10-8
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.821 A=0.055 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF092725=Hepsin-SRCR=PU(3.6=26.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGAGGAACCTGGGGCTCAC
R:
TTGGTACAGTGGCTCCTGGTC
Band lengths:
101-203
Functional annotations
There are 1 annotated functions for this event
PMID: 15627507
Both exons together encode a transmembrane domain and cause cytosolic retention when skipped. Hepsin/-TM (skipping) overexpression does not affect the growth of a human cancer cell line.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: