MmuEX6009970 @ mm9
Exon Skipping
Gene
ENSMUSG00000001249 | Hpn
Description
hepsin [Source:MGI Symbol;Acc:MGI:1196620]
Coordinates
chr7:31888519-31894211:-
Coord C1 exon
chr7:31894110-31894211
Coord A exon
chr7:31893984-31894025
Coord C2 exon
chr7:31888519-31888648
Length
42 bp
Sequences
Splice sites
3' ss Seq
CTGTTCTCCCCTCTCCACAGTGA
3' ss Score
11.37
5' ss Seq
AAGGTGAGT
5' ss Score
10.47
Exon sequences
Seq C1 exon
GTGGCCGGACTGCAGCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCATTGTGGGTACCCTGCTGTTCCTGACAGGCATTGGGGCCGCGTCCTGGGCCATTG
Seq A exon
TGACCATCCTACTGCAGAGTGACCAGGAGCCACTGTACCAAG
Seq C2 exon
TGCAGCTCAGTCCAGGGGACTCACGGCTTGCGGTGTTTGACAAGACGGAGGGAACGTGGAGGCTACTGTGCTCCTCACGCTCCAATGCCAGGGTGGCAGGGCTCGGCTGTGAGGAGATGGGCTTTCTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000001249-'10-10,'10-8,11-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.055 A=0.000 C2=0.006
Domain overlap (PFAM):
C1:
NO
A:
PF092725=Hepsin-SRCR=PU(3.6=26.7)
C2:
PF092725=Hepsin-SRCR=FE(39.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAGCTCTCATTGTGGGTAC
R:
GCACAGTAGCCTCCACGTTC
Band lengths:
136-178
Functional annotations
There are 1 annotated functions for this event
PMID: 15627507
Both exons together encode a transmembrane domain and cause cytosolic retention when skipped. Hepsin/-TM (skipping) overexpression does not affect the growth of a human cancer cell line.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: