MmuEX0030356 @ mm10
Exon Skipping
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67093441-67094863:+
Coord C1 exon
chr11:67093441-67093586
Coord A exon
chr11:67093667-67093757
Coord C2 exon
chr11:67094474-67094863
Length
91 bp
Sequences
Splice sites
3' ss Seq
TTCTCTGTGTCCTGCCACAGGAA
3' ss Score
10.08
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
Exon sequences
Seq C1 exon
CTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAAAGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACAAGCAACAGCTAGACGAGAGGCTCAAGAA
Seq A exon
GAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAG
Seq C2 exon
GCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-'26-34,'26-30,37-34=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.041 A=0.000 C2=0.417
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=PD(0.5=2.0),PF0157614=Myosin_tail_1=PD(53.6=75.5),PF0003816=Filament=FE(17.6=100),PF0157614=Myosin_tail_1=PU(4.3=28.6)
A:
PF0003816=Filament=FE(11.0=100),PF0157614=Myosin_tail_1=FE(9.2=100)
C2:
PF0003816=Filament=FE(47.4=100),PF0157614=Myosin_tail_1=FE(39.4=100),PF104734=CENP-F_leu_zip=PU(42.0=44.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGAGCTCCCTCGAACAAGA
R:
TCTTCCTCCAGCTCCTCGATG
Band lengths:
170-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types