Special

MmuEX0030356 @ mm9

Exon Skipping

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66906943-66908365:+
Coord C1 exon
chr11:66906943-66907088
Coord A exon
chr11:66907169-66907259
Coord C2 exon
chr11:66907976-66908365
Length
91 bp
Sequences
Splice sites
3' ss Seq
TTCTCTGTGTCCTGCCACAGGAA
3' ss Score
10.08
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
Exon sequences
Seq C1 exon
CTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAAAGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACAAGCAACAGCTAGACGAGAGGCTCAAGAA
Seq A exon
GAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAG
Seq C2 exon
GCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-'28-28,'28-27,29-28
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.041 A=0.000 C2=0.415
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0157614=Myosin_tail_1=PD(0.1=0.0),PF0157614=Myosin_tail_1=PD(25.4=69.4),PF0003816=Filament=FE(17.6=100),PF0157614=Myosin_tail_1=PU(1.6=28.6)

							
A:
PF0003816=Filament=FE(11.0=100),PF0157614=Myosin_tail_1=FE(3.5=100)

							
C2:
PF0003816=Filament=FE(47.4=100),PF0157614=Myosin_tail_1=FE(15.0=100),PF104734=CENP-F_leu_zip=PU(41.5=45.4)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000007301





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr17:10638873-10638963 
HIGH PSI
HsaEX6021091
(MYH3)
Human
(hg19)
chr17:10542190-10542280 
HIGH PSI
HsaEX6021091
(MYH3)
Rat
(rn6)
chr10:53637097-53637187 
Cow
(bosTau6)
chr19:30237496-30237586 
ALTERNATIVE
BtaEX6087219
(MYHC-EMBRYONIC)
Chicken
(galGal4)
chr18:545100-545190 
Chicken
(galGal3)
chr18:598775-598865 
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GGAGAGCTCCCTCGAACAAGA

				
R: 
TCTTCCTCCAGCTCCTCGATG

				
Band lengths: 
170-261

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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