MmuEX0032533 @ mm10
Exon Skipping
Gene
ENSMUSG00000059857 | Ntng1
Description
netrin G1 [Source:MGI Symbol;Acc:MGI:1934028]
Coordinates
chr3:109827627-109839179:-
Coord C1 exon
chr3:109839120-109839179
Coord A exon
chr3:109832468-109832635
Coord C2 exon
chr3:109827627-109827761
Length
168 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTCCTTGCAAGACT
3' ss Score
5.41
5' ss Seq
TAGGTCAGT
5' ss Score
6.39
Exon sequences
Seq C1 exon
TTGCTCCCAAATTAGCTTTGTCAACAGTTTCTTCTGTTCAAGTTGCAAACCACAAGCGAG
Seq A exon
ACTGTGAATGCTTCGGCCACTCCAATCGGTGCAGTTATATCGATCTGCTAAACACAGTCATTTGCGTGAGCTGTAAACACAACACTAGAGGGCAGCACTGTGAGTTATGCAGGCTGGGCTACTTCAGAAATGCTTCTGCACAGCTGGACGATGAGAATGTGTGCATAG
Seq C2 exon
AGTGTTATTGTAACCCTTTGGGCTCAATCCATGATCGTTGTAATGGCTCAGGATTTTGTGAGTGTAAGACTGGAACAACAGGGCCTAAATGTGATGAGTGTCTGCCAGGAAATTCCTGGTACTACGGCTGTCAAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000059857-'23-18,'23-17,24-18
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0005319=Laminin_EGF=WD(100=94.7)
C2:
PF0005319=Laminin_EGF=PU(91.7=95.7),PF079748=EGF_2=PU(5.6=4.3)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTCCCAAATTAGCTTTGTCA
R:
GTTGACAGCCGTAGTACCAGG
Band lengths:
194-362
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.738, FDR=0.000), PC9 Depleted at 14 days (FC=0.778, FDR=0.008), Late Xenograft Depleted (FC=0.772, FDR=0.007).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types