BtaEX0022181 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000014530 | MGC139448
Description
Bos taurus netrin G2 (NTNG1), mRNA. [Source:RefSeq mRNA;Acc:NM_001080369]
Coordinates
chr3:35969787-36050479:-
Coord C1 exon
chr3:36050453-36050479
Coord A exon
chr3:36028787-36028954
Coord C2 exon
chr3:35969787-35971035
Length
168 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTCCTTGCAAGATT
3' ss Score
7.43
5' ss Seq
TAGGTCAGT
5' ss Score
6.39
Exon sequences
Seq C1 exon
GTATCCCCAGTATTTCCAGTATTGGTA
Seq A exon
ATTGTGAATGCTTCGGCCACTCCAATCGATGCAGTTATATCGATCTGCTAAACACAGTCATTTGCGTGAGCTGTAAACACAACACTAGAGGGCAGCACTGTGAGTTATGCAGGCTGGGCTACTTCAGAAACGCTTCTGCACAGCTGGACGATGAGAATGTGTGCATAG
Seq C2 exon
CGAATGTCTGCGACAACGAGCTCCTGCACTGCCAGAACGGAGGGACGTGCCACAACAACGTGCGCTGCCTGTGCCCAGCCGCGTACACGGGCATCCTGTGCGAGAAGCTGCGGTGCGAGGAGGCGGGCAGCTGCGGCCCCGACTCCGGCCAGGGCGCGCCCCCGCGCGGCTCCCCCGCGCTGCTGTCGCTGCTGGCCGCTCTGCTGGGGACAGCCGGCCCCCTGGGCATCTAGGCGGCGCACCCAGCCACCAGACGGGCCGTGCTCTGGGGAAGCAAACACAGCCCCAGCATTTGCTACTAACATAGGAAACCCGCTCTCAGACACTCCCACTCAAACACAGTGTACAAAGAAGGCCTAACTGAACTAAGCCATATTTATCAGCCGTGGACAGCACACCTGAGTCAAGACTGTTAATTTCTGACTCCCAGAGGAGTTGGCAGCTGTTGATATTATCACTGCGAATCACATTGCCAGCTGCAGAGCTTACCACCCATCGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014530_MULTIEX1-3/4=C1-C2
Average complexity
ME(2-3[98=100])
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.065
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF079748=EGF_2=WD(100=39.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCCCAGTATTTCCAGTATTGGT
R:
CGCCTAGATGCCCAGGGG
Band lengths:
260-428
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.738, FDR=0.000), PC9 Depleted at 14 days (FC=0.778, FDR=0.008), Late Xenograft Depleted (FC=0.772, FDR=0.007).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development