RnoEX0061341 @ rn6
Exon Skipping
Gene
ENSRNOG00000031136 | Ntng1
Description
netrin G1 [Source:RGD Symbol;Acc:1563465]
Coordinates
chr2:212746524-212771566:-
Coord C1 exon
chr2:212771540-212771566
Coord A exon
chr2:212751540-212751707
Coord C2 exon
chr2:212746524-212746658
Length
168 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTCCTTGCAAGACT
3' ss Score
5.41
5' ss Seq
TAGGTCAGT
5' ss Score
6.39
Exon sequences
Seq C1 exon
GTATCCCCAGCATTTCCAGTATTGGTA
Seq A exon
ACTGTGAATGCTTCGGCCACTCCAATCGGTGCAGTTATATCGATCTGCTAAACACAGTCATTTGCGTGAGCTGTAAACACAACACTAGAGGGCAGCACTGTGAGTTATGCAGGCTGGGCTACTTCAGAAATGCTTCTGCACAGCTGGACGATGAGAATGTGTGCATAG
Seq C2 exon
AGTGTTATTGTAACCCTTTGGGCTCAATCCATGATCGTTGTAATGGCTCAGGATTTTGTGAGTGTAAGACTGGAACGACAGGGCCTAAATGTGATGAGTGTCTGCCAGGAAATTCCTGGTACTACGGCTGTCAAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000031136-'5-8,'5-7,8-8
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0005319=Laminin_EGF=WD(100=94.7)
C2:
PF0005319=Laminin_EGF=PU(88.0=95.7),PF079748=EGF_2=PU(5.6=4.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTATCCCCAGCATTTCCAGTA
R:
GTTGACAGCCGTAGTACCAGG
Band lengths:
162-330
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.738, FDR=0.000), PC9 Depleted at 14 days (FC=0.778, FDR=0.008), Late Xenograft Depleted (FC=0.772, FDR=0.007).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]