GgaEX0002304 @ galGal3
Exon Skipping
Gene
ENSGALG00000001896 | NTNG1
Description
NA
Coordinates
chr8:918246-964306:+
Coord C1 exon
chr8:918246-918272
Coord A exon
chr8:935682-935849
Coord C2 exon
chr8:963654-964306
Length
168 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTCCTTGAAAGACT
3' ss Score
6.55
5' ss Seq
TAGGTCAGT
5' ss Score
6.39
Exon sequences
Seq C1 exon
GTATACCCAGTATTTCCAGTATTGGTA
Seq A exon
ACTGTGAATGCTTCGGGCACTCCAACCGGTGCAGTTACATCGAGCTGCTCAATACGGTCATTTGCGTGAGCTGTAAGCACAACACTAGAGGTCAGCACTGTGAGTTATGCAGGCTGGGCTACTTCAGAAATGCTTCTGCAGAGCTGGACGATGAAAATGTGTGCATAG
Seq C2 exon
CAAATGTGTGTGACAATGAACTCCTGCACTGCCAGAATGGAGGGACATGCATCAACAATGTGCGGTGCCAGTGCCCTCCGGCCTACACTGGCATCTTATGCGAGAAGCTTAAGTGTGAAAGGGATCCAGGAGGCTGCAGCCAAAGCTCCGCCCAGGGGGCAATGTCACACTGTCCCTTATTACTGCTGATGGCCCTACTAGGAGCAGCTGGGGTTCACATGTGCTAGGCTTCGTATGGTCAACGGCATCGTATTTGGACTGCTTCAAAATAACGTGCCACACGAAAAGAAAACAAAATCCAAGCATTTGCTACCGAAATTTCAAAAAGGAAAAAGAGAGAAAAAAATACCACATACAAACTAGGAAGGCCTAACTGAACTAAGCCATATTAATCAGTTACGGACAGCGCTCTGAGTCAAGACTGCTCACCACAACCGGAGATGGGCTGGCAGCTCCTGCTCTGCCCAACCAGTGCCGTCTGCCAGGCTCACATAGGACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000001896_MULTIEX1-6/7=2-C2
Average complexity
C2
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0005319=Laminin_EGF=WD(100=94.7)
C2:
PF0005319=Laminin_EGF=PD(6.1=3.9),PF079748=EGF_2=PD(91.7=43.4)
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATACCCAGTATTTCCAGTATTGGT
R:
CTAGCACATGTGAACCCCAGC
Band lengths:
252-420
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.738, FDR=0.000), PC9 Depleted at 14 days (FC=0.778, FDR=0.008), Late Xenograft Depleted (FC=0.772, FDR=0.007).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]