MmuEX0032533 @ mm9
Exon Skipping
Gene
ENSMUSG00000059857 | Ntng1
Description
netrin G1 [Source:MGI Symbol;Acc:MGI:1934028]
Coordinates
chr3:109585600-109654709:-
Coord C1 exon
chr3:109654683-109654709
Coord A exon
chr3:109635386-109635553
Coord C2 exon
chr3:109585600-109585829
Length
168 bp
Sequences
Splice sites
3' ss Seq
TTTCTGTTCTTCCTTGCAAGACT
3' ss Score
5.41
5' ss Seq
TAGGTCAGT
5' ss Score
6.39
Exon sequences
Seq C1 exon
GTATCCCCAGCATTTCCAGTATCGGTA
Seq A exon
ACTGTGAATGCTTCGGCCACTCCAATCGGTGCAGTTATATCGATCTGCTAAACACAGTCATTTGCGTGAGCTGTAAACACAACACTAGAGGGCAGCACTGTGAGTTATGCAGGCTGGGCTACTTCAGAAATGCTTCTGCACAGCTGGACGATGAGAATGTGTGCATAG
Seq C2 exon
CTAATGTCTGCGACAATGAGCTCCTGCACTGCCAGAATGGAGGGACCTGCCAGAACAATGTGCGCTGCGCGTGCCCAGACGCCTACACCGGCATCCTCTGTGAGAAGCTACGGTGCGAAGAGGCGGGCAGCTGTGGCTCCGAATCCGGCCAGGGAGCACCCCCGCGGGGCTCCCCAGCACTGCTGCTGCTGACCATGCTGCTGGGGACTGCCGGTCCCCTGGTGTTCTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000059857_MULTIEX1-5/6=2-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
Show structural model
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.015
Domain overlap (PFAM):
C1:
NO
A:
PF0005319=Laminin_EGF=WD(100=94.7)
C2:
PF0005319=Laminin_EGF=PD(6.2=3.9),PF079748=EGF_2=PD(91.7=42.9)
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCCAGCATTTCCAGTATCGG
R:
GCAGTCCCCAGCAGCATG
Band lengths:
234-402
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.738, FDR=0.000), PC9 Depleted at 14 days (FC=0.778, FDR=0.008), Late Xenograft Depleted (FC=0.772, FDR=0.007).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types