Special

MmuEX0038892 @ mm9

Exon Skipping

Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136567618-136572662:+
Coord C1 exon
chr5:136567618-136567679
Coord A exon
chr5:136571397-136571478
Coord C2 exon
chr5:136572531-136572662
Length
82 bp
Sequences
Splice sites
3' ss Seq
TGTCCCCGTTTCACTCCCAGGGA
3' ss Score
10.74
5' ss Seq
TCGGTGAGG
5' ss Score
8.99
Exon sequences
Seq C1 exon
CCGGGACGATGTGATCGGGAAGGTCTGCCTCACGAGGGACGCGCTAGCCTCTCACCCAAAGG
Seq A exon
GGACTTAGCACCAAAAGATCGGAATGGCGCATCTGACCCTTTTGTCCGAGTGCACTACAATGGCCGGACCCAGGAGACCTCG
Seq C2 exon
GTGGTGAAGAAATCCTGCTACCCACGCTGGAATGAGACATTTGATTTTGAGCTGGAAAAAGGAGCCTCAGAAGCACTGCTGGTGGAGGCCTGGGACTGGGATCTTGTTAGCCGGAATGACTTTCTAGGCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952_MULTIEX3-2/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.177 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0016825=C2=PD(11.0=40.9)

							
A:
PF0016825=C2=FE(32.9=100)

							
C2:
PF0016825=C2=FE(52.4=100)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000120203





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000037869, ENSMUSP00000098136
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
No conservation detected
Human
(hg19)
chr7:102143610-102143691 
ALTERNATIVE
HsaEX0052381
(RASA4B)
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
chr25:35074613-35074694 
HIGH PSI
BtaEX6110402
([1])
Chicken
(galGal4)
chr19:4172367-4172448 
HIGH PSI
GgaEX6017116
([2])
Chicken
(galGal3)
chr19:4138546-4138627 
HIGH PSI
GgaEX6017116
([3])
Zebrafish
(danRer10)
chr5:58658742-58658823 
HIGH PSI
DreEX6013880
(rasa4)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GATGTGATCGGGAAGGTCTGC

				
R: 
TCCGGCTAACAAGATCCCAGT

				
Band lengths: 
170-252

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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