DreEX6013880 @ danRer10
Exon Skipping
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58656558-58659042:+
Coord C1 exon
chr5:58656558-58656690
Coord A exon
chr5:58658742-58658823
Coord C2 exon
chr5:58658908-58659042
Length
82 bp
Sequences
Splice sites
3' ss Seq
ACTTTCGGCTTTATTTCTAGAGA
3' ss Score
7.99
5' ss Seq
TCGGTAAGG
5' ss Score
10.48
Exon sequences
Seq C1 exon
GTGTTGACGGATGGATGAACCTAACAGAGATTGACCCAGATGAGGAGGTGCAAGGGGAGATTCACCTCCAGATCTCAGTGCTGGGAGATGGAGATATCCCACGGAAACTGTGCTGCCAGGTCCTAGAGGCAAG
Seq A exon
AGATCTTGCGAAAAAGGATCGCAACGGGGCCTCTGATCCTTTTGTCCGAGTGAGATACAATGGGAAAACGTATGAGAGCTCG
Seq C2 exon
GTGGTTAAAAAGTCCTGTTATCCTCGCTGGAACGAGAGTTTTGAGTTTGAACTGGACGAGGCGCTGACTGACAGTCTACTGAGTGTGGAGGTGTGGGACTGGGATCTGGTCAGCAGAAATGACTTCCTGGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372-'5-7,'5-5,6-7=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.022 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0016825=C2=PU(9.6=17.8)
A:
PF0016825=C2=FE(32.5=100)
C2:
PF0016825=C2=FE(53.0=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAGTGCTGGGAGATGGAGA
R:
TCTGCTGACCAGATCCCAGTC
Band lengths:
177-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]