Special

MmuEX0039593 @ mm10

Exon Skipping

Gene
ENSMUSG00000040146 | Rgl3
Description
ral guanine nucleotide dissociation stimulator-like 3 [Source:MGI Symbol;Acc:MGI:1918996]
Coordinates
chr9:21981604-21987904:-
Coord C1 exon
chr9:21987851-21987904
Coord A exon
chr9:21987499-21987713
Coord C2 exon
chr9:21981604-21981743
Length
215 bp
Sequences
Splice sites
3' ss Seq
CCACACACCCCTCTGTTCAGGGC
3' ss Score
5.75
5' ss Seq
CAGGTAAGG
5' ss Score
11.08
Exon sequences
Seq C1 exon
GGTAGACAGCAAGAGGACAGAGGGACAAGATCTGAACTTCAGCAAGAACCTGAG
Seq A exon
GGCTGTAGTTTCGGTGCTGGGTTCTTGGCTGCGAAATCATCCTCAGGACTTCCGGGATCCCCCTGATCATCAGAACCTAGGCAATGTCCGAATCTTTTTGGGCTGGGCAGCCCCAGGAGGGGCAGAAGCCAGGGAAGCAGAGAAACTTCTAGAAGATTTCTTGAAGGAGGCTAAGGGAGAGCAGACAGAGGAGGAAAAGCGACTGGCTTGGTCAG
Seq C2 exon
GCCCTCCTAGGATTGCCCAGACCCCCGGATCGGAGTTTGCGGAGGACTGTGTGGAAGAGGAAGGCCCCAGTTCAGAAGGCCCAGAGCTCCTGGACTTCAGCGTGGATGACGTGGCAGAGCAGCTGACCCTTATGGATGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000040146_CASSETTE3
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.720 A=0.550 C2=0.596
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0061815=RasGEF_N=FE(16.5=100)

							
A:
PF0061815=RasGEF_N=PD(29.4=43.8)

							
C2:
PF0061714=RasGEF=PU(7.7=34.0)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000035726





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000148965, ENSMUSP00000150195
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr19:11415947-11416114 
ALTERNATIVE
HsaEX0053325
(RGL3)
Human
(hg19)
chr19:11526623-11526790 
ALTERNATIVE
HsaEX0053325
(RGL3)
Rat
(rn6)
chr8:22998528-22998742 
HIGH PSI
RnoEX6034944
(Rgl3)
Cow
(bosTau6)
chr7:17021031-17021245 
HIGH PSI
BtaEX6038208
(RGL3)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
chr6:7991235-7991404 
HIGH PSI
DreEX6020287
(rgl3a)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GACAGCAAGAGGACAGAGGGA

				
R: 
ACATCCATAAGGGTCAGCTGC

				
Band lengths: 
189-404

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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