MmuEX0041592 @ mm10
Exon Skipping
Gene
ENSMUSG00000063531 | Sema3e
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [Source:MGI Symbol;Acc:MGI:1340034]
Coordinates
chr5:14210291-14226530:+
Coord C1 exon
chr5:14210291-14210384
Coord A exon
chr5:14224287-14224406
Coord C2 exon
chr5:14225553-14226530
Length
120 bp
Sequences
Splice sites
3' ss Seq
CCTCTGGGTCGCCTTTGCAGGGA
3' ss Score
8.62
5' ss Seq
AAGGTAGAA
5' ss Score
6.33
Exon sequences
Seq C1 exon
GAACCCCTGTTTCACCTGGAGTCACACAGATCTGAGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAACTCCTCCTTTGTGTCCACGCTAGTTG
Seq A exon
GGAATGAGCTGTTTGCTGGACTCTACAGTGACTATTGGGGCAGAGACTCGGCGATCTTCCGCAGCATGGGGAAGTTAGGCCATATTCGCACTGAGCATGACGATGAGCGGCTCCTGAAAG
Seq C2 exon
AACCAAAATTTGTAGGTTCATATATGATTCCTGATAACGAAGACCGAGATGACAACAAAATGTACTTTTTCTTTACTGAGAAGGCGCTGGAGGCGGAGAACAACGCCCACACGATCTACACCCGAGTGGGGCGGCTGTGCGTGGTGAGGCTGGCGCGACCATGGCGTTATGCTTTATTTCCTCAGTTTACCTCCCTCACCTCCCTCTGTATAGCCAGGCCTCTGTTATATTGTTTTACATTGTTTTACCATTTCGTTGGTTCTTTTGAGAGTTCCATACAGCATGCTTTGATTATATTTGTGTCCCGTCCCTCAACTTTTCCACGGTCCACAGCCCTTCCTTACACACACAGCTCTGTGTCCTCTTATAGTCTTCAACTAGAAATACATAACATCTTTGTTTGTTTGTTTGGTCGGTTGGTTGGTTTTTTGAGACAGGGTTTCTGTCTCAAAAAGTATTGAGAGTGTTTGAATGAAAGCACTGCACTGAGGGGTTGAAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000063531-'10-12,'10-11,11-12
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.094 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(7.0=100)
A:
PF0140314=Sema=FE(9.0=100)
C2:
PF0140314=Sema=FE(10.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACCCCAACTCCTCCTTTGT
R:
AGAGGGAGGTGAGGGAGGTAA
Band lengths:
242-362
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types