MmuEX0041597 @ mm10
Exon Skipping
Gene
ENSMUSG00000028064 | Sema4a
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:MGI Symbol;Acc:MGI:107560]
Coordinates
chr3:88452971-88455739:-
Coord C1 exon
chr3:88455548-88455739
Coord A exon
chr3:88454679-88454846
Coord C2 exon
chr3:88452971-88453131
Length
168 bp
Sequences
Splice sites
3' ss Seq
GAAAGTGCCTGTTTCCTCAGAAC
3' ss Score
5.27
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
Exon sequences
Seq C1 exon
ACTCGAGGTAGAAATTCTGGTTTCCCTGGCCTGAAACCCACACTGGCTCTGGCAGGATGGGTGCCTGGAGGGCCGCGCGCCCGTTCCAGCCAGGCTGAGCCTGCTTGCCTTGCCTGGACCCCCTTCTTTGCCTCGGATGGCACCCTGATTCAGGGTTTGAACACAGGACTTCTCAGTGTGGCTGGCTCAAGG
Seq A exon
AACCATCTGGTGACCATCTCAGGCTGACCATGGCCCTACCATCCCTGGGCCAGGACTCATGGAGTCTCCTGCGTGTTTTTTTCTTCCAACTCTTCCTGCTGCCATCACTGCCACCTGCTTCTGGGACTGGTGGTCAGGGGCCCATGCCCAGAGTCAAATACCATGCTG
Seq C2 exon
GAGACGGGCACAGGGCCCTCAGCTTCTTCCAACAAAAAGGCCTCCGAGACTTTGACACGCTGCTCCTGAGTGACGATGGCAACACTCTCTATGTGGGGGCTCGAGAGGCCGTCCTGGCCTTGAATATCCAGAACCCAGGAATCCCAAGGCTAAAGAACATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028064-'5-20,'5-18,13-20
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref, Alt. ATG (>10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.220 C2=0.002
Domain overlap (PFAM):
C1:
NA
A:
NO
C2:
PF0140314=Sema=PU(72.0=66.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAAACCCACACTGGCTCTG
R:
GTTGCCATCGTCACTCAGGAG
Band lengths:
245-413
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types