MmuEX6000066 @ mm10
Exon Skipping
Gene
ENSMUSG00000006740 | Kif5b
Description
kinesin family member 5B [Source:MGI Symbol;Acc:MGI:1098268]
Coordinates
chr18:6225768-6226968:-
Coord C1 exon
chr18:6226864-6226968
Coord A exon
chr18:6226382-6226430
Coord C2 exon
chr18:6225768-6225823
Length
49 bp
Sequences
Splice sites
3' ss Seq
TGACTTTTCTTTCTGCTTAGGTT
3' ss Score
10.56
5' ss Seq
ATGGTAAGT
5' ss Score
11.01
Exon sequences
Seq C1 exon
GGTAAACTTCATGATCCAGAAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAATTATATTTACTCCATGGATGAAAATTTGGAATTTCATATTAAG
Seq A exon
GTTTCATATTTTGAAATATATTTGGATAAGATAAGGGACTTGTTAGATG
Seq C2 exon
TTTCAAAGACTAACCTTTCAGTCCATGAAGACAAAAACCGTGTTCCCTATGTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000006740-'12-13,'12-12,13-13
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.114 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0022518=Kinesin=FE(10.9=100)
A:
PF0022518=Kinesin=FE(5.1=100)
C2:
PF0022518=Kinesin=FE(5.8=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATGATCCAGAAGGCATGGGA
R:
AGGGAACACGGTTTTTGTCTTCA
Band lengths:
144-193
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types