MmuEX6013069 @ mm9
Exon Skipping
Gene
ENSMUSG00000007279 | Scube2
Description
signal peptide, CUB domain, EGF-like 2 [Source:MGI Symbol;Acc:MGI:1928765]
Coordinates
chr7:116972549-116974758:-
Coord C1 exon
chr7:116974642-116974758
Coord A exon
chr7:116974311-116974394
Coord C2 exon
chr7:116972549-116972653
Length
84 bp
Sequences
Splice sites
3' ss Seq
TTCCACGTTCTGTCTATCAGGAC
3' ss Score
9.44
5' ss Seq
GGGGTAATT
5' ss Score
5.8
Exon sequences
Seq C1 exon
AAGTGAAGGGCTTCCCGCCCACTAGCATGACACCGCGTGTGTCCCTGCACTGTGGTAAAAGTGGTGGAGGAGACAGGTGCTTCCTCAGATGTCGCTCTGGCATTCACCTCTCGTCAG
Seq A exon
GACTGCAAGAGGCTTACTCTGTCACCTGTGGCTCTTCCTCTCTCAGAAGCAAACAGCAAAAATCAAATGACTCTGCCTTTGGGG
Seq C2 exon
ATGTCGTCACCGTCAGGACAAGTGTAACCTTTAAGCTAAATGAAGGCAAGTGTAGTTTGCAAAAGGCCAAGCTGTCTCCCGAGGGTCTACGACCAGCACTACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000007279-'12-13,'12-12,13-13=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.216
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTCCCTGCACTGTGGTAAA
R:
TAGACCCTCGGGAGACAGCTT
Band lengths:
168-252
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: