DreEX0064628 @ danRer10
Exon Skipping
Gene
ENSDARG00000037362 | scube2
Description
signal peptide, CUB domain, EGF-like 2 [Source:ZFIN;Acc:ZDB-GENE-050302-80]
Coordinates
chr7:28318645-28321960:-
Coord C1 exon
chr7:28321844-28321960
Coord A exon
chr7:28319645-28319728
Coord C2 exon
chr7:28318645-28318749
Length
84 bp
Sequences
Splice sites
3' ss Seq
ACCTTTACCTTTATCTGTAGGAG
3' ss Score
9.9
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
Exon sequences
Seq C1 exon
AGGCAGAGGACTCTCCACTAGTGCCACCTCCTGCCAGGCCCACTCTCAACTGCAATAAGCAGGGAGGAGGCGAGCTCTGCTACCTAACCTGCCAGTCACAGGTCCACATCAGCAGCG
Seq A exon
GAGCTGAGGATTCATACACAGTGACCTGTGGCATGCCGCTGCCGTGCCACACAGGAGGACAGTGGAATGGATCATATTGCCCAG
Seq C2 exon
GTTCGGGAATCAAAACCATTGCCACATTTAAATCTGGACAGGGGAAGTGCAATCTAAAGCGGAGTCATGAAAATCTTGCACATAGCTTCAAAACTGCCCTGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037362-'13-17,'13-16,14-17
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.083
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGGACTCTCCACTAGTGCC
R:
GATTGCACTTCCCCTGTCCAG
Band lengths:
167-251
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]