GgaEX1051264 @ galGal3
Exon Skipping
Gene
ENSGALG00000005892 | SCUBE2
Description
NA
Coordinates
chr5:10470250-10474417:+
Coord C1 exon
chr5:10470250-10470372
Coord A exon
chr5:10471612-10471698
Coord C2 exon
chr5:10474214-10474417
Length
87 bp
Sequences
Splice sites
3' ss Seq
CTGTATCGTCTGTCTGTTAGGAA
3' ss Score
7.31
5' ss Seq
TGGGTAATT
5' ss Score
6.19
Exon sequences
Seq C1 exon
ACATTAATGAATGTAGCATCAACAACGGTGGCTGCCAACAGCTGTGTGTGAACACACCGGGAGACTATGAATGCCTGTGCCATTCTAACTACAAATTACACTGGAATAAAAAGGATTGTGTTG
Seq A exon
GAACACAAGATGCCTACACTCTCGCCTGTGGCAGCTCATCTCAACTTAAGAAAAAGCAGCAAAATACAAATGCATCCACTTGGCTGG
Seq C2 exon
ACACGTGTGATGTGCGCTGCGCCCGAAAAAAGACAGAGAAGAGGTTCCGTAAAACCATCCGAACCTTACGGAAGACGGTCAGCAGGGACCAGTTCCGCGTCCGTGTCTCTGGCATGGACCACGAAGTGGCCAGGAAGTCCCTCAAGCTGTCGGAGCCACAGCAGTCGTGCGGTGTGGGACAGATGCATGTGGGTAGCAGATGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000005892-'11-15,'11-11,13-15=AN
Average complexity
A_C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.101
Domain overlap (PFAM):
C1:
PF146701=FXa_inhibition=WD(100=85.7)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCCAACAGCTGTGTGTGAA
R:
CTGCTGACCGTCTTCCGTAAG
Band lengths:
177-264
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]