GgaEX1051264 @ galGal4
Exon Skipping
Gene
ENSGALG00000005892 | SCUBE2
Description
signal peptide, CUB domain, EGF-like 2 [Source:HGNC Symbol;Acc:HGNC:30425]
Coordinates
chr5:8944919-8949086:+
Coord C1 exon
chr5:8944919-8945041
Coord A exon
chr5:8946281-8946367
Coord C2 exon
chr5:8948883-8949086
Length
87 bp
Sequences
Splice sites
3' ss Seq
CTGTATCGTCTGTCTGTTAGGAA
3' ss Score
7.31
5' ss Seq
TGGGTAATT
5' ss Score
6.19
Exon sequences
Seq C1 exon
ACATTAATGAATGTAGCATCAACAACGGTGGCTGCCAACAGCTGTGTGTGAACACACCGGGAGACTATGAATGCCTGTGCCATTCTAACTACAAATTACACTGGAATAAAAAGGATTGTGTTG
Seq A exon
GAACACAAGATGCCTACACTCTCGCCTGTGGCAGCTCATCTCAACTTAAGAAAAAGCAGCAAAATACAAATGCATCCACTTGGCTGG
Seq C2 exon
ACACGTGTGATGTGCGCTGCGCCCGAAAAAAGACAGAGAAGAGGTTCCGTAAAACCATCCGAACCTTACGGAAGACGGTCAGCAGGGACCAGTTCCGCGTCCGTGTCTCTGGCATGGACCACGAAGTGGCCAGGAAGTCCCTCAAGCTGTCGGAGCCACAGCAGTCGTGCGGTGTGGGACAGATGCATGTGGGTAGCAGATGTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000005892_MULTIEX1-2/4=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.101
Domain overlap (PFAM):
C1:
PF146701=FXa_inhibition=WD(100=85.7)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCCAACAGCTGTGTGTGAA
R:
CTGCTGACCGTCTTCCGTAAG
Band lengths:
177-264
Functional annotations
There are 1 annotated functions for this event
PMID: 32249828
[CRISPR screen]. This alternative exon is implicated in cell fitness by a CRISPR-based exon deletion phenotypic screen in RPE-1 cells.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]