MmuEX6015927 @ mm10
Exon Skipping
Gene
ENSMUSG00000063531 | Sema3e
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E [Source:MGI Symbol;Acc:MGI:1340034]
Coordinates
chr5:14224287-14226530:+
Coord C1 exon
chr5:14224287-14224406
Coord A exon
chr5:14225553-14225695
Coord C2 exon
chr5:14226416-14226530
Length
143 bp
Sequences
Splice sites
3' ss Seq
ATGTTTGTTTTTTTCTGAAGAAC
3' ss Score
6.9
5' ss Seq
GTGGTGAGG
5' ss Score
6.13
Exon sequences
Seq C1 exon
GGAATGAGCTGTTTGCTGGACTCTACAGTGACTATTGGGGCAGAGACTCGGCGATCTTCCGCAGCATGGGGAAGTTAGGCCATATTCGCACTGAGCATGACGATGAGCGGCTCCTGAAAG
Seq A exon
AACCAAAATTTGTAGGTTCATATATGATTCCTGATAACGAAGACCGAGATGACAACAAAATGTACTTTTTCTTTACTGAGAAGGCGCTGGAGGCGGAGAACAACGCCCACACGATCTACACCCGAGTGGGGCGGCTGTGCGTG
Seq C2 exon
AATGACATGGGAGGACAGAGAATCCTGGTGAACAAGTGGAGCACTTTCCTTAAAGCGCGGCTGGTTTGCTCAGTGCCGGGAATGAATGGAATCGACACATACTTTGACGAACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000063531-'2-15,'2-12,13-15=AN
Average complexity
A_C2
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(9.0=100)
A:
PF0140314=Sema=FE(10.6=100)
C2:
PF0140314=Sema=FE(8.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGTTTGCTGGACTCTACAGT
R:
AGTTCGTCAAAGTATGTGTCGA
Band lengths:
226-369
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types