MmuEX6036156 @ mm10
Exon Skipping
Gene
ENSMUSG00000019428 | Fkbp8
Description
FK506 binding protein 8 [Source:MGI Symbol;Acc:MGI:1341070]
Coordinates
chr8:70527724-70531109:+
Coord C1 exon
chr8:70527724-70527836
Coord A exon
chr8:70529391-70529675
Coord C2 exon
chr8:70530940-70531109
Length
285 bp
Sequences
Splice sites
3' ss Seq
ACACTTGCCTGTCCCCTTAGGGC
3' ss Score
10.86
5' ss Seq
TGGGTAAGT
5' ss Score
10.24
Exon sequences
Seq C1 exon
AATGGGTGCGCGGGGGCGGGGCTGCGTATCTGAAGCTGTTTCCCGAGGCGCGGCGGCCGCGGCTGGGGGCGGGGAGGGGGCACAGGACCCCGAGCGGGGTCCCCGAGCCGCAG
Seq A exon
GGCCAGTTCCTGATCCCAGCAGCATGGCGTCTTGGGCTGAGCCCTCTGAGCCTGCTGCCCTGCGACTTCCTGGGGCCCCACTGCTGGAGGGCTTTGAGGTGCTCGATGGGGTTGATGATGCAGAGGAGGAAGATGACCTAAGTGGGCTGCCACCACTAGAGGACATGGGACAGCCTACAGTGGAGGAGGCTGAGCAGCCTGGAGCCCTGGCTCGGGAGTTCCTGGCAGCCACAGAGCCTGAGCCAGCCCCAGCCCCAGCCCCGGAAGAGTGGCTGGACATTCTGG
Seq C2 exon
GGAACGGATTGCTGCGGATGAAGACACTGGTCCCAGGCCCGAAAGGCTCTAGCCGCCCACTCAAGGGCCAGGTGGTGACCGTTCACCTGCAGATGTCCCTGGAGAATGGCACCCGTGTACAGGAAGAGCCTGAACTGGCCTTCACGCTGGGAGACTGCGATGTTATCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000019428-'0-6,'0-3,9-6
Average complexity
C1
Mappability confidence:
93%=100=83%
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.898 C2=0.474
Domain overlap (PFAM):
C1:
NA
A:
NO
C2:
PF0025423=FKBP_C=PU(45.6=71.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCGGGGCTGCGTATCTGAA
R:
CTGGATAACATCGCAGTCTCCC
Band lengths:
268-553
Functional annotations
There are 2 annotated functions for this event
PMID: 19546213
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: affinity chromatography technology, anti tag coimmunoprecipitation, colocalization, competition binding, detection by mass spectrometry, fluorescence polarization spectroscopy, fluorescent resonance energy transfer, glutathione s tranferase tag, isothermal titration calorimetry, mutation analysis, protein array, pull down, subcellular preparation. ELM ID: ELMI002479; ELM sequence: LPPLE; Overlap: FULL
PMID: 23413029
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: affinity chromatography technology, anti tag coimmunoprecipitation, colocalization, competition binding, detection by mass spectrometry, fluorescence polarization spectroscopy, fluorescent resonance energy transfer, glutathione s tranferase tag, isothermal titration calorimetry, mutation analysis, protein array, pull down, subcellular preparation. ELM ID: ELMI002479; ELM sequence: LPPLE; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types