Special

MmuEX6071977 @ mm9

Exon Skipping

Gene
ENSMUSG00000028064 | Sema4a
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:MGI Symbol;Acc:MGI:107560]
Coordinates
chr3:88239881-88242262:-
Coord C1 exon
chr3:88242105-88242262
Coord A exon
chr3:88241725-88241822
Coord C2 exon
chr3:88239881-88241174
Length
98 bp
Sequences
Splice sites
3' ss Seq
TGCTTGCTTTGTCTTCTCAGGCC
3' ss Score
9.31
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
GGTGCAGTGTTTGCAGGCTTCTCTGGAGGCATCTGGAGAGTTCCCAGGGCCAATTGCAGTGTCTACGAGAGCTGTGTGGACTGTGTGCTTGCCAGGGACCCTCACTGTGCCTGGGACCCTGAATCAAGACTCTGCAGCCTTCTGTCTGGCTCTACCAA
Seq A exon
GCCTTGGAAGCAGGACATGGAACGCGGCAACCCGGAGTGGGTATGCACCCGTGGCCCCATGGCCAGGAGCCCCCGGCGTCAGAGCCCCCCTCAACTAA
Seq C2 exon
TTAAAGAAGTCCTGACAGTCCCCAACTCCATCCTGGAGCTGCCCTGCCCCCACCTGTCAGCACTGGCCTCTTACCACTGGAGTCATGGCCGAGCCAAAATCTCAGAAGCCTCTGCTACCGTCTACAATGGCTCCCTCTTGCTGCTGCCGCAGGATGGTGTCGGGGGCCTCTACCAGTGTGTGGCGACTGAGAACGGCTACTCATACCCTGTGGTCTCCTATTGGGTAGACAGCCAGGACCAGCCCCTGGCGCTGGACCCTGAGCTGGCGGGCGTTCCCCGTGAGCGTGTGCAGGTCCCGCTGACCAGGGTCGGAGGCGGAGCTTCCATGGCTGCCCAGCGGTCCTACTGGCCCCATTTTCTCATCGTTACCGTCCTCCTGGCCATCGTGCTCCTGGGAGTGCTCACTCTCCTCCTCGCTTCCCCACTGGGGGCGCTGCGGGCTCGGGGTAAGGTTCAGGGCTGTGGGATGCTGCCCCCCAGGGAAAAGGCTCCACTGAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028064-'35-37,'35-36,36-37=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.471 C2=0.178
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0143720=PSI=PU(61.4=66.0)

							
A:
PF0143720=PSI=PD(36.8=61.8)

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000029700





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000103155, ENSMUSP00000103156, ENSMUSP00000125909, ENSMUSP00000128510, ENSMUSP00000128887
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr1:156175556-156175656 
ALTERNATIVE
HsaEX6033962
(SEMA4A)
Human
(hg19)
chr1:156145347-156145447 
HIGH PSI
HsaEX6033962
(SEMA4A)
Rat
(rn6)
chr2:187801367-187801464 
HIGH PSI
RnoEX6010212
(Sema4a)
Cow
(bosTau6)
chr3:14660948-14661048 
HIGH PSI
BtaEX6014456
(SEMA4A)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
chr19:24800760-24800848 
HIGH PSI
DreEX6073049
(sema4ab)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
AGTGTTTGCAGGCTTCTCTGG

				
R: 
GAGTTGGGGACTGTCAGGACT

				
Band lengths: 
181-279

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]










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