BtaEX6014456 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000012228 | SEMA4A
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A [Source:HGNC Symbol;Acc:HGNC:10729]
Coordinates
chr3:14659269-14663272:-
Coord C1 exon
chr3:14663115-14663272
Coord A exon
chr3:14660948-14661048
Coord C2 exon
chr3:14659269-14660572
Length
101 bp
Sequences
Splice sites
3' ss Seq
TTTTTGCTTTCTCTCCTCAGGAA
3' ss Score
12.5
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
GGAGCAGTGTTTGTAGGCTTCTCAGGAGGCATCTGGAAGGTTCCTCGAGCCAACTGTAGTGTCTACGAGAGCTGTATGGACTGTGTCCTTGCCCGGGACCCTCACTGTGCCTGGGACCCTGAGTCTCAAACCTGCCGCCTCCTGCCCACCCCCATCCT
Seq A exon
GAAGTCCTGGAAACAGGACATGCAGCAAGGGAACCCAGAGTGGGCATGTGCCAGTGGCCCCATGGGCAGGAGTCTGGGGCCCAGGAGCCGCCCCCAAATCA
Seq C2 exon
TTAAAGAAGTCCTGGCTGTCCCCAACTCCATCCTGGAGCTCCCCTGTCCCCAGAGCTCGGCTCTGGCCTCTTACCACTGGAGTCACGGGGTAGAAGCCATCCCAGAGGCCCCTTCCACAGTCTACAATGGCTCCCTCTTGCTGCTTCTGCGAGATGGGGCGGGGGGTCTCTATCAGTGCTGGGCCACTGAGAATGACTTCTCGTACCCTGTGGTCTCCTACTGGGTGCACAGTCAGGACCAGCCCCTCGCCCTGGACCCCAAACTGGCGGGCATTCCCCGGGAGCGCATGGAGGCCCCGCTGACCAGGGTTGGCGGCGGGGCCGCCCTGGCTGCCCCGAAGTCCTACTGGCCCCACTTCCTCACCGTCACTGTGCTCCTTGCCCTGGTGCTCTCAGGAGCCCTCGTCACCTTCCTTGTATCCCCACTGGGGGCACTCCGAGCCCGGGGCAAAGTCCAAGGCTGTGGGACCCTGCCCTCCAGGGAGAAAGCCCCACTGAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000012228-'19-20,'19-18,20-20=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.029 C2=0.193
Domain overlap (PFAM):
C1:
PF0143720=PSI=PU(66.0=66.0)
A:
PF0143720=PSI=PD(32.1=48.6)
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGTAGGCTTCTCAGGAGGCA
R:
CTTCTACCCCGTGACTCCAGT
Band lengths:
244-345
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]