MmuEX6073865 @ mm10
Exon Skipping
Gene
ENSMUSG00000053965 | Pde5a
Description
phosphodiesterase 5A, cGMP-specific [Source:MGI Symbol;Acc:MGI:2651499]
Coordinates
chr3:122760878-122776345:+
Coord C1 exon
chr3:122760878-122760967
Coord A exon
chr3:122774553-122774624
Coord C2 exon
chr3:122776256-122776345
Length
72 bp
Sequences
Splice sites
3' ss Seq
CTTTTCTCTTCTTATCCTAGGTT
3' ss Score
10.64
5' ss Seq
AAGGTAACA
5' ss Score
8.92
Exon sequences
Seq C1 exon
GATCCCCGGTTCAATGCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGTATCCTTTGTATGCCAATTAAGAACCACAGGGAAGAG
Seq A exon
GTTGTTGGTGTAGCTCAGGCCATCAACAAGAAATCAGGAAATGGTGGGACCTTCACTGAAAAAGATGAAAAG
Seq C2 exon
GACTTTGCGGCCTACCTGGCATTCTGTGGTATTGTTCTTCACAATGCTCAGCTCTATGAAACGTCATTGCTGGAGAACAAAAGAAATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000053965-'6-6,'6-5,9-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.042 C2=0.000
Domain overlap (PFAM):
C1:
PF0159021=GAF=FE(19.2=100)
A:
PF0159021=GAF=FE(15.2=100)
C2:
PF0159021=GAF=PD(7.9=40.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCCCCGGTTCAATGCAGAAG
R:
TGTTCTCCAGCAATGACGTTTCA
Band lengths:
168-240
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types