MmuINT0032741 @ mm10
Intron Retention
Gene
ENSMUSG00000027330 | Cdc25b
Description
cell division cycle 25B [Source:MGI Symbol;Acc:MGI:99701]
Coordinates
chr2:131193239-131193687:+
Coord C1 exon
chr2:131193239-131193412
Coord A exon
chr2:131193413-131193594
Coord C2 exon
chr2:131193595-131193687
Length
182 bp
Sequences
Splice sites
5' ss Seq
CCTGTTAGT
5' ss Score
-5.28
3' ss Seq
CCATCTGCCATGCTTTCCAGAAG
3' ss Score
7.62
Exon sequences
Seq C1 exon
GATCTCATCATGTTCAGCAAGTGCCAGAGGCTCTTCCGCTCCCCATCCATGCCATGCAGTGTGATCCGACCCATCCTCAAGAGGCTAGAGCGGCCCCAGGACCGGGATGTGCCTGTCCAGAGCAAGCGCAGGAAAAGTGTGACACCCCTGGAAGAGCAGCAGCTTGAAGAACCT
Seq A exon
GTTAGTTCCTTCCTCCACAGGCTGGGCGGTCACTGAAGATGCTCTGGGTGTACCCTCTTAGCTAGAGGTGTAGCAGTGGGTAGTGGGCAGGAGGTAGTTTAAGTGGCAGAGAAAGAAGGTCTCAACCAAATAGCACTGAGAGGCAGTCTCTTCCCTGACCCTCCATCTGCCATGCTTTCCAG
Seq C2 exon
AAGGCCCGTGTCTTTCGCTCAAAGTCGCTGTGTCATGAGATTGAGAACATCCTGGATAGTGACCACCGTGGACTGATCGGAGATTACTCTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000027330:ENSMUST00000028804:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.374 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF066178=M-inducer_phosp=FE(21.1=100)
A:
NA
C2:
PF066178=M-inducer_phosp=PD(5.9=51.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTTCAGCAAGTGCCAGAGG
R:
AATCTCCGATCAGTCCACGGT
Band lengths:
250-432
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types