MmuINT0040527 @ mm9
Intron Retention
Gene
ENSMUSG00000025650 | Col7a1
Description
collagen, type VII, alpha 1 [Source:MGI Symbol;Acc:MGI:88462]
Coordinates
chr9:108862210-108862681:+
Coord C1 exon
chr9:108862210-108862356
Coord A exon
chr9:108862357-108862558
Coord C2 exon
chr9:108862559-108862681
Length
202 bp
Sequences
Splice sites
5' ss Seq
CACGTAGGA
5' ss Score
3.74
3' ss Seq
TTGTAATGCTTTCTCTTAAGCGC
3' ss Score
5.78
Exon sequences
Seq C1 exon
GTGGCCCTATGAAACACCGGATACTCCCAGGAAACAAAGAGTCTGCAGAGATACGGGATCTCGAAGGAGGAGTCAGCTACTCCGTGAGAGTGACGGCGCTTGTTGGAGACCGAGAGGGTGCACCGGTGTCCATTGTCATCACCACAC
Seq A exon
GTAGGAGGGTGTTTGGGTTGGAAGAAGTCTTGGAGTGAGGCTGTGTTCAAGGGGCTTTTGGGGAAGGATCTGTGCTGGTGTTGGAGAGGGATCAAGGATTTATAGTGAGCCTGTGTGGGTAAAGTTCGGGGAAGAAATGTATTCAGAGGGGTTGGTTAGCCTAGAGGGCAGAGCCTCCCTGATTGTAATGCTTTCTCTTAAG
Seq C2 exon
CGCCTGCGACCCCAGCACTTCTGGAGACTCTTCAAGTTGTGCAGAGCGGGGAGCACTCCTTGAGGCTGCGATGGGAACCAGTACCCGGAGCACCAGGCTTCCGCCTGCACTGGCAACCTGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025650-Col7a1:NM_007738:19
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.340 A=NA C2=0.286
Domain overlap (PFAM):
C1:
PF0004116=fn3=PD(51.9=82.0)
A:
NA
C2:
PF0004116=fn3=PU(45.6=85.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCCTATGAAACACCGGAT
R:
CTGGTGCTCCGGGTACTG
Band lengths:
242-444
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: