Special

MmuINT0078349 @ mm10

Intron Retention

Gene
ENSMUSG00000035697 | Arhgap45
Description
Rho GTPase activating protein 45 [Source:MGI Symbol;Acc:MGI:1917969]
Coordinates
chr10:80022077-80022355:+
Coord C1 exon
chr10:80022077-80022187
Coord A exon
chr10:80022188-80022274
Coord C2 exon
chr10:80022275-80022355
Length
87 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGC
5' ss Score
8.07
3' ss Seq
TCTGTCCCTGCCTCCCTTAGCCC
3' ss Score
8.36
Exon sequences
Seq C1 exon
CCCCACATGCCTCTCTTGTCCATCTACTCACTGGCCCTGGAACAAGATTTGGAGTTTGGCCACGGCATGGTGCAGGCAGCGGGCACACTGCAGACCCAGACCTTCATGCAG
Seq A exon
GTGGGCTGTGCCTGAGGTAAGGTGGCCTGCCCGCTGCAGATGCCACCCACCAGTTTGGCACTCACAGTCTGTCCCTGCCTCCCTTAG
Seq C2 exon
CCCCTGACGCTGCGGCGGTTGGAGCATGAGAGGCGCAGGAAGGAGATCAAAGAATCTTGGCATCGAGCACAGAGGAAGCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035697:ENSMUST00000099501:9
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.108 A=NA C2=1.000
Domain overlap (PFAM):

C1:
PF0061118=FCH=PD(34.4=83.8)
A:
NA
C2:
NO


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
ALTERNATIVE
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCATCTACTCACTGGCCCT
R:
CAGCTTCCTCTGTGCTCGATG
Band lengths:
175-262
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types