MmuINT0094243 @ mm10
Intron Retention
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123510645-123511039:-
Coord C1 exon
chr4:123510946-123511039
Coord A exon
chr4:123510800-123510945
Coord C2 exon
chr4:123510645-123510799
Length
146 bp
Sequences
Splice sites
5' ss Seq
GCTGTAAGT
5' ss Score
8.56
3' ss Seq
TTATTCTTTTTCTGTCACAGGAT
3' ss Score
11.4
Exon sequences
Seq C1 exon
GCTAGAATTGCTGCTGCAGATTGCAAACAAGATACAGAATGGTGCTTTGAACTGTGAAGAAAAACTGACACTAGCTAAGAACACACTGCAGGCT
Seq A exon
GTAAGTGTTGTTACATGCGTTCCGCTTTTCACCCAGCTGCTCTGACAGTCAAAATCCAGCAGTGTTTCTTGCTTTCTCTTGGTTTTCTCCATCTTAGTGGGTGAGAGCAATAGAGGTTTCTCACCCTTATTCTTTTTCTGTCACAG
Seq C2 exon
GATGCTGCACACCTGGAATCAGGACAGCCAGTGCAGTGTGAGTCAGATGTCATTATGTACATTCAAGAGTGTGAGGGTCTCATCAGGCAGCTGCAGGTGGATCTCCAGATCCTACGGGATGAAAAGTACTACCAGTTAGAAGAGCTTGCCTTTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649:ENSMUST00000082108:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCAGATTGCAAACAAGATACAGA
R:
CTAAAGGCAAGCTCTTCTAACTGGT
Band lengths:
234-380
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types