MmuINT0094262 @ mm10
Intron Retention
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123539796-123544831:-
Coord C1 exon
chr4:123544742-123544831
Coord A exon
chr4:123539874-123544741
Coord C2 exon
chr4:123539796-123539873
Length
4868 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGC
5' ss Score
8.05
3' ss Seq
TCCCTTTTCTCCCTTTGCAGCCC
3' ss Score
12.72
Exon sequences
Seq C1 exon
GTCCGCAAGCACATCAATGATCTCTATGAAGACCTTCGGGATGGACACAACCTGATCTCCCTGTTAGAGGTCCTCTCAGGCATCAAACTG
Seq A exon
GTGAGCTTCTTTCTTCTAACGAGAGTCCTGTAAGCAAGGCTTCCTGTAGCAGTAGTTTAGTGCTTGCTCCATTGGGCGGGAGAGAGAAGTAGATGAGTTATTGATAACACTTTGTTTCTCACTCAGGCATATAGTTTACAGGTGACATCATCTCAATTTATACATTATGTATTGTCATGTGAGGTCTCTGTACCCAAGTCCTAAGGATTTAACTTGAGACTTCACACCTGTTAGAAGGCATTGTGTAATTAAATTATGTCTCCAGCTTTAGGTCTCATGCCTTGATAAAATCAGAAATACTTTTATTTTGTACCTCCGGACACCTTCTTAGTCATTCCTCATCTCTCTCTAGATACTAATCTTTCAGAAACATTATATTCATGTTTAAAAGTTTTTGGGGGAATAAATTGCATTTTTCTTTGTATAGGTTTTTTTTCTAGTTTGTATAGACTTCCCTTTTCTCTATTATGTTTTTGTCTTTTTCTCCTAATTTTCTGTGTTTAGCTAAGCATTTGAAGTTTTTAAGAACCCAGGTGTTTGGAGTTTCTCCTACTTTCCTCCATGATTTGTTGAGTCCCACAAATGTGGAAAAATTCTGGCTTGGTCTGAGCGAGCCTGTCCTCATAAAGCAGTGTTTACACACAGTCTCCTTACATATGCTGGCCTGTCCCAGTGCTTGTGGGAAGCCCTTTCCTACAGAGAATCCTGGCTGAGACCCCTGACCCTTCAAAGAATACCTCTCACACTTGCTTTGTTTAAGGGATTAAAATAACATTCCTTTGGCCATTGTTTCAAATATCTAAGCACAATGGAAGCAAGTGTTCTTTGTGTGTGGTTCAGTGTCTTTCTTTCAGATGCTATAGGCAGTGCAGTTTTCATTGGAAACATCCATGCTTGAGTATTAGAAGCATGGGTGTGTGGGGGGGAGATCTGCAGAGGCACACAGTGCAAGCACTCTAAATCTTAGCCCACTCAATGCTTCTGGTGGATTCTTAGTCATTGTTTGTTGTGTTAAAGAATAGTTTCTTTGGCACACTAGATTTCATTGTTTATGTGATGTTCAACTCCTTCTAGGATTGGGGCTTAGCTTCCTGAGCCCTTTGTTTTGGGTTTACTTTAAAAACAAAAAACTCTTAGTAGTACAGTTATTCATATGTATTTTTTAGTGGTCAGAATTGTCACAAGTGCTGGGTGTGGTGGCGCACACCTTTGATCCCAGCACTTGGGAGGCAGAGGCAGGCAAATTTCTGAGTTCGAGGCCAGTCTGGTCTACAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAAAAAACCAAACCAAACAAAACAAAAACAACAACAAACAGAATTGCCATAAGCCTTTGTTAGCATAGCTAGTATGTGTAATTCCTGTTTCTCGTCTGGCCTTCATTCATTTTATTATTTTTTTTAAGATTATTTTAAAGATTTATTTATATATTTTATGGAGTACTCTATGTGCATGTGTACCTGTGTGTCAGAAGAGGGCATCGGATCTTTACATATGGTTGTGAGCCATCATGTAGGTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGCCAGTGCTTTGAACCACTGACTGGCCTTCATTTATTGATCTAGTAAGATATCAGAATTCCAATCTTCTCTGCTGGACGTTATTCTTCAGCACTCATTCAGTGCATCTCCTACAGTTGATAGGTGTGTCAACAGTGGTTTTCACATTGGAAAGTCATTCTTTCTGATGATACTGTGTACCATGCAGGGTAAAGGTGTTCCCATTTAACTGTTCTTCTTAACCTTTGAAGACGTGGGTGTTTATGGAGATGCTCATATTATCTTTTCACCAGAAAGCATCTGATGTCTTTCAGAGTTGTTCTTGTCATAGTTCCAACCATAACAGAATTACAAATGTAGGATGGGTAGACATGAAGAATTGAAGATGTTCTCAAAAGAAGAAGTGTTCAGTGTCCTCTGCTCTGCTGTTGATCTGACTTTTTTTCTTTCTTTTGGAGGCGGGAGGTTTTTTCATTTTTAGATAAAAAACATTTATTCATTTCTCCTTTGTGTGTGTGTATGCGTGTATGTGTGCTTTCTTTTCTGTTCCTCTATTGTGTTTTTTTTGGTTGGTGGCCAAAGGAGCCAGCAGGGCTGAAGACCCTTCGTCTCGTGAGCATGCCCTCCTGGCGTCATGCAAACAGCGAGGATCAGGAAGAGGAAGATGATGATGATGTAGTAGGTCTTTTGGGGATGCTAGCATCCCAGCTGGCACTTCAGGGCCATCAGAGAGGCCAGACTTCTCAAGGTCTCCAAGTAACTTGGTCACCAGTTGGCTTCTCCTTCCACCAGGGAATATGGGTCCCAAAGAAAGTGGTTAAAATTAATTGTGCCTATTGCTACTGTTAGATGATCCTGAAGTGGCAATTATTTTGCTTATCTGAGAATGGTTGGCACAAAACCAATCATAGTACATGGGAGTATCAAGTCCTTGTGACCTGGAGACCTATGGCTTTCTGCTGGCCTTGTTGTATTGTCTGATTCTGACTGCTGAACTGTGGAAGGGTTAGCTGACAGCATGAAAATTAATTGCTCACGATTCCACAATATAGACTAACGGTATTTTCTCTCCACTTCTAGCCTCTGGTTTTTCACTAAGCACGTGTCAATTGTTTTCGAAATTGAGTGTTGTTTGCAAACTCACTGCATGAGATACTCTGGCCTTGGTCAGTAATCTCACTAGTTACTGTAAGGAACAGAGATTTAATTTGCATGTTCTTGGGCTATTAACTATTGGTGGAGGGTCCATTAAGAATAGAGATTAATGTTTTAAGAGGAGCCTTTTCTGCTTATAGGTTCTAAATTAGAACCCATAATCTTTCTCCAGAAATGGGACAATACCCACAACCTATGCCCTCTTATAGTCATCTCTTCATCTCCCTTCCCATTGTCTTACAATTGTATTTAGACTGCCATTCTCTAACCTGTATTCTCCATTGATCTGAAATAACTTAGAAAAGGTTCTTGCTGAAAAAAAGGTCCCTAAAGCTCACAGAAGAAGTTACCTTGTTATACAGAAAGGCCTTGGTATCTCTGCAAATTATCCCATTTGGAGAATGTTGTTTAGAAATGCTGGAGTGTACCCGAAGGGACACTCTTAGCCCTCTCCATATGTTGGGCTACTTTTTGGCTGTTATAAGATTTTCTTATTGCAGATCCCACCACAGAAGTACCTTATATTTGTTCAATCTTTAATTAGCAGTGCCAATTTAATATTTACAAATGAATTGGGTGCAATCTGATGAGGGTGATATTTCAGTCCCAGCTTTAGGAAAACCAAGCAGCACTGCAGGAGATGTGAGAGCACACGCCTGTTCTGAGAGCACTTGCTGGGCAGGCACACTTTCCTTACCTCCCCATAGGCATGGCTGAGCCTTGGGCAGGGCTGACAGTTAACAGATGGAGGTGGCTTTGCTTTGTTTTGTAGTATGACCCAAGGATTAAATAGCAGAAACAGTTTTATTTGTGATTCTTTTTTTTTTTTTAAAGATTTATTTATTTATTATATGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGCGTCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCCTGACCTTCGGAAGAGCAGTCGGGTGCTCTTACCCACTGAGCCATCTCACCAGCCCTATTTGTGATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAGGCGTGCACCACCATGCCCAGCTCTAATGGTGGTTCTTTTTTTTTTTTTTTAAAGACTTTTTATTTATGTATATATATATTTATGTATATATGTAAGTACACTGTAGCTGTCTTCAGACACACCAGAAGAGGGAGTCAGATCTTGTTACGGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCGGGTGCTCTTACCCACTGAGCCATCTCACCAGCCCCCTTGTGATTCTTAAATAACTATCACCATTACAATTTTATTGCTCTGAGAATTTCATTTGGGGATCAGCAGCTTTCTATGGTAACACTCACCTCCTTGAGAGATGGGTTATTTCCTAGATTCTGTCATCCATTTTGGAAGTTTTCCACTTTTCTTGAGCACCAGTAACTGCATTCTCTGTATTTAAATTTGCCTTTCTAGAGTTTGCCTTCTCTAGCCAGGTTTGTGCTGAGGTTGGTTCTACCCATTACTAATTATGCAGTTAGACTTTCTGCCTTTCCTCTGCAGTGTAGATAATTGTGATTTTTTTTTTTTTTAAGATTTGTTTTATTTATATGTGTACACTGTCACTTTCTTCAGACACCCCAGAAGAGGGCACTGGATCCCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGAGCTCAGGAAGAGCAGCCAGCCAGTGCTCTTAACCACTGAGCCATCTCTCCAGCCCCGATAATTGTGATTTTAAGACTCATAGATCTATACCCTGTGTTACTGTTAACCCTACACCCCAAATAAACCTCTGCCACACACAGAAGAGCCTGTGCAGAGGTGACTCTGACACAGCATTAGACTCAGATAAGCTTGTGGGTTTGTGTGATGTAGAGTGGTGTGCAAGCATTGTCAGCTGCTGAGAGCCTGCTGGGCACATGTAGGCCCTGCTCTTCAAGGGGCTTCCTTTAATGCTCCCACTCCTGGCTTCAGCCATGAGCAGTTCTGTGTGCTCTTTTAGTCCTAGGAAAGGCTGTGCAGGGGTAAATTACCATAGCTAGAATTAACATCCCTTTTCTCCCTTTGCAG
Seq C2 exon
CCCAGAGAGAAGGGCAGGATGCGTTTCCACAGGCTGCAGAATGTGCAGATCGCCCTGGACTTCCTAAAGCAGCGGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649:ENSMUST00000082108:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.015 A=NA C2=0.012
Domain overlap (PFAM):
C1:
PF0030726=CH=PU(33.3=96.7)
A:
NA
C2:
PF0030726=CH=FE(24.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types