MmuINT0097610 @ mm10
Intron Retention
Gene
ENSMUSG00000024593 | Megf10
Description
multiple EGF-like-domains 10 [Source:MGI Symbol;Acc:MGI:2685177]
Coordinates
chr18:57275681-57277123:+
Coord C1 exon
chr18:57275681-57275827
Coord A exon
chr18:57275828-57276988
Coord C2 exon
chr18:57276989-57277123
Length
1161 bp
Sequences
Splice sites
5' ss Seq
GAAGTAAGT
5' ss Score
9.82
3' ss Seq
CTATGAGTTTCTATTTGCAGTCT
3' ss Score
6.32
Exon sequences
Seq C1 exon
GTGTGCACTGTGACAGTGTGTGCGCTGAGGGACGCTGGGGTCCTAACTGCTCGCTGCCCTGCTACTGTAAAAATGGGGCTTCGTGTTCTCCGGATGATGGCATCTGTGAGTGTGCACCCGGATTCCGAGGCACCACTTGCCAGAGAA
Seq A exon
GTAAGTGTCTCCTACATGATGCTTTCTGCCCCCACCCCTCGTGTCCCCTTTCCTGGGAATTATGTACAGAATCATCTCTGCCCCACCCCTTAGTTCCTAGTGACATTTTGAGGCAGCTGAGGGAAGAGTCCTTGAAGAACTGTCCTCAAATAACACTTCCCCAAGAATTTTACCCTTTCCAACCTCCAGAACCAACTTAACAGATGGGGGCGTTAACCCTTTCTTCTTAGTGACTATTTCTTTACCTCATTGGCATGATCATCAGGACACGTTTCTCAATTCCTCCTTGGAGTGCCCAAATGGTGTTCCAGAAATCCACTGACCTGTAAATATTTTATTCGGCAATTTGGCATCAGCCTCACCTCAATTACCAGAAGCCAACATCAGCCAAACCTTGAGGCAATCACACACCTCCCTTAGTAAGCTGAAAGTCAGCGCCCTGGGGAAAGCAGAATTGAAAGTTAAAAATGGAGACCACTGATGTTGCTCCGTAGGAACCTCGAACAAAGACATGATCTGCAACAGTTCACTAAATCTTTCAGTGCATGTAACCAAGAAAGAATATGTAACTGGTTATAACATGCACTGTTGTAATTTCTCAAATCAGCTGATTTTTTCCATTAAAGAGTTCTATGCCATCATTCTATATTTAAAACCAAATAAAGCCTTTGGAAGTACCCAGATCCATAGCGTTCTGTTCCTCACCCACTCTAATCTAATCACAGGCATTCACCTGTACTTTTTTTTTAAGAAGAAATATCTTCAAACCTCATGTGTCTATCGTTTTTTGCCAATCTTGATTCTTGAAGAATAATGGATCATTCAACAATAACTCAGCAATGCTTAATTGAGATACTGATATTTAGAGTTTCATTTTCCCCATTTCCTTATTTGTTCTACAAAACGTCTTCGAGCCCCTGGTCTAGGGCTGGCCTTGAGTCAACCGCTGGAAGATAGTGGTATACGGAAGACATCCCCTCACCCCAGGGAAATCTGGACTCTAGTGGCTTCTTCCCTTGTTAAAAGCTGCCTTCTTGTTTCATGCCTGAAAAGTCCCTATTACCGGTTAGAGTTTTCTTATTCCCCCAGTTTCAGTAAACCTCTGTGTGGAGCAGGAGATAAACCCAACTTGGGGAAGTAACTATGAGTTTCTATTTGCAG
Seq C2 exon
TCTGCTCCCCCGGTTTTTATGGACATCGCTGTAGCCAGACCTGCCCGCAGTGTGTGCACAGCAGTGGGCCCTGCCACCACATCACGGGCCTGTGTGACTGCTTACCTGGCTTCACCGGTGCCCTGTGCAATGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000024593:ENSMUST00000075770:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF126612=hEGF=PD(23.1=6.0)
A:
NA
C2:
PF126612=hEGF=WD(100=28.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTGTGACAGTGTGTGCGC
R:
GCACCGGTGAAGCCAGGTAA
Band lengths:
262-1423
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types