MmuINT0097657 @ mm10
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:154253715-154254279:+
Coord C1 exon
chr4:154253715-154253837
Coord A exon
chr4:154253838-154254108
Coord C2 exon
chr4:154254109-154254279
Length
271 bp
Sequences
Splice sites
5' ss Seq
CGCGTGAGC
5' ss Score
4.09
3' ss Seq
GCCTGTGTGTTCATCCCCAGCCC
3' ss Score
8.8
Exon sequences
Seq C1 exon
ATGTTGACGAGTGTGCCTCTGGTCACAGCGGCTGCGAACACCACTGTTCTAATCTGGCTGGCTCCTTCCAGTGCTTCTGTGAGGCTGGCTACCGGCTGGATGAGGACCGCAGGGGCTGCACGC
Seq A exon
GTGAGCCTCCCCCTACGCTTCCCTACCTTCTGGGTGTCTGTTCCCAGTAGCCCACACTGTCTGTCTCCTGCTGTAGGGGTGGGGCCAGCAGGCTGGTTGGGTTCATGATTGATAGGCTAGGACACAGTGTTACGGGGGGGGGGGGGGAGTGGGGGGTGGAGGGTGGGGGTGGGGTCTTGAGCATCCCATTTCAAGAGCTGCCGACCTATGACGGTGCTGTTGCTGAGTCAGTGGGCGGCAAAGGCTGAGAGGCCTGTGTGTTCATCCCCAG
Seq C2 exon
CCCTAGAAGAATCCGTGGTGGACCTGGATGGCCAGCTGCCCTTTGTCCGGCCCCTGCCCCACATTGCGGTGCTGGGGGATGAGCTTCCACAACTCTTCCAAGATGACTATGGGGCTGAGGAGGAGGCTGTGGCGGAGCTGCGGGGAGAACACACTCTCACTGAGAAGTTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751:ENSMUST00000030897:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.069
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=95.2)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTTGACGAGTGTGCCTCTG
R:
AACTTCTCAGTGAGAGTGTGTTCT
Band lengths:
292-563
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types